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PMID: 6419025 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Negative complementation of recA protein by recA1 polypeptide: in vivo recombination requires a multimeric form of recA protein.

Molecular & general genetics : MGG ·Vol. 193 ·No. 1 ·1984-00-00 ·Pages 53-7

Yancey SD, Porter RD

Abstract

Recombination in vivo was studied in recA- heterozygous lacZ merodiploids by performing beta-galactosidase assays after infection with lambda precA+. Recombination as measured by beta-galactosidase production was a linear function of lambda pecA+ multiplicity of infection (MOI) when the strain contained a deletion of the chromosomal recA gene. However, when the strain carried a recA1 missense allele, a higher lambda precA+ MOI was required to obtain levels of recombination comparable to the delta (recA) strain, and the slope of the dose-response curve increased to approximately two. It is proposed that negative complementation occurs in mixed tetramers of wild-type and missense recA polypeptides, and that in vivo recombination is a property of a multimeric form of recA protein.

MeSH Terms
Alleles Escherichia coli/genetics Galactosidases/genetics Genes, Bacterial Genetic Complementation Test Genotype Rec A Recombinases/genetics Recombination, Genetic beta-Galactosidase/genetics
Chemicals
Rec A Recombinases Galactosidases beta-Galactosidase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Yancey S D
Porter R D
References (23)
23 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00327413
Published
1984-00-00
Pages
53-7
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Grants
NIGMS NIH HHS · GM-26422 · United States
External Links
PubMed source DOI: 10.1007/BF00327413
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