Home LiteratureArticle Details
PMID: 6420394 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Purification of a Mycoplasma pneumoniae adhesin by monoclonal antibody affinity chromatography.

Journal of bacteriology ·Vol. 157 ·No. 2 ·1984-02-00 ·Pages 678-80

Leith DK, Baseman JB

Abstract

A 165,000-dalton surface protein of Mycoplasma pneumoniae, designated protein P1, appears to be the major attachment ligand of the pathogen. We employed monoclonal antibody affinity chromatography to obtain purified protein P1.

MeSH Terms
Adhesins, Bacterial Adhesiveness Antibodies, Monoclonal Bacterial Proteins/isolation & purification Chromatography, Affinity/methods Molecular Weight Mycoplasma pneumoniae/physiology
Chemicals
Adhesins, Bacterial Antibodies, Monoclonal Bacterial Proteins adhesin, Mycoplasma pneumoniae
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Leith D K
Baseman J B
References (13)
13 references, click to expand
  1. Tissue cultures and mycoplasmas.
    Tex Rep Biol Med. 1965 Jun;23:Suppl 1:285+ PMID: 5833547
  2. Characteristics of virulent, attenuated, and avirulent Mycoplasma pneumoniae strains.
    J Bacteriol. 1969 Nov;100(2):1037-43 PMID: 5359607
  3. A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels.
    Eur J Biochem. 1974 Jul 1;46(1):83-8 PMID: 4850204
  4. Surface parasitism by Mycoplasma pneumoniae of respiratory epithelium.
    J Exp Med. 1977 May 1;145(5):1328-43 PMID: 870608
  5. The effects of organic solvents and temperature on the desorption of yeast 3-phosphoglycerate kinase from immunoadsorbent.
    J Immunol Methods. 1979;25(4):375-81 PMID: 372447
  6. Isolation of binding sites to glycophorin from Mycoplasma pneumoniae membranes.
    Infect Immun. 1980 Dec;30(3):628-34 PMID: 6785229
  7. An ELISA to detect monoclonal antibodies specific for lipid determinants of Mycoplasma pneumoniae.
    J Immunol Methods. 1983 Nov 11;64(1-2):165-78 PMID: 6196412
  8. Mycoplasma pneumoniae proteins that selectively bind to host cells.
    Infect Immun. 1982 Jul;37(1):382-6 PMID: 6809635
  9. Molecular basis for cytadsorption of Mycoplasma pneumoniae.
    J Bacteriol. 1982 Sep;151(3):1514-22 PMID: 6809731
  10. Mycoplasma pneumoniae adhesin localized to tip structure by monoclonal antibody.
    Nature. 1982 Aug 19;298(5876):765-7 PMID: 7110314
  11. Host discrimination of Mycoplasma pneumoniae proteinaceous immunogens.
    J Exp Med. 1983 Feb 1;157(2):502-14 PMID: 6401796
  12. Inhibition of mycoplasma pneumoniae hemadsorption and adherence to respiratory epithelium by antibodies to a membrane protein.
    Infect Immun. 1983 Mar;39(3):1180-6 PMID: 6404820
  13. Mycoplasma pneumoniae infection: role of a surface protein in the attachment organelle.
    Science. 1982 Apr 16;216(4543):313-5 PMID: 6801766
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.157.2.678-680.1984
Published
1984-02-00
Pages
678-80
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC215303
Subset
IM
Grants
NIAID NIH HHS · AI 18540 · United States
External Links
PubMed source DOI: 10.1128/jb.157.2.678-680.1984 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2