Home LiteratureArticle Details
PMID: 6420790 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Localization of the structural gene for human apolipoprotein A-I on the long arm of human chromosome 11.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 81 ·No. 2 ·1984-01-00 ·Pages 508-11

Cheung P, Kao FT, Law ML, Jones C, Puck TT, Chan L

Abstract

Apolipoprotein A-I (apo A-I), the major apolipoprotein in human high density lipoproteins, is involved in the disease atherosclerosis. Cloned apo A-I cDNA (pA1-3) was used as a probe in chromosome mapping studies to detect the human apo A-I structural gene sequence in human-Chinese hamster cell hybrids. Southern blot analysis of 13 hybrids localized the gene to human chromosome 11. Confirmation of the chromosomal assignment was obtained by analysis of a hybrid (J1) containing a single human chromosome, no. 11. Regional mapping was achieved by using deletion subclones of J1 that localized the human apo A-I structural gene to the region 11q13 leads to qter. Since the human apolipoprotein C-III (apo C-III) structural gene is closely linked to apo A-I, it can be assigned to the same region on the long arm of chromosome 11. By extension of methods previously described, it now appears possible to carry out fine-structure analysis of this and related gene regions on chromosome 11 and to study the biochemical concomitants of these genes and of genes on other chromosomes for analysis of their role in atherosclerosis.

MeSH Terms
Animals Apolipoprotein A-I Apolipoproteins/genetics Chromosome Deletion Chromosome Mapping Chromosomes, Human, 6-12 and X Cricetinae Humans Hybrid Cells Nucleic Acid Hybridization
Chemicals
Apolipoprotein A-I Apolipoproteins
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Cheung P
Kao F T
Law M L
Jones C
Puck T T
Chan L
References (42)
42 references, click to expand
  1. Phosphorylation changes induced by cAMP derivatives in the CHO cell and selected mutants.
    Exp Cell Res. 1982 Nov;142(1):63-8 PMID: 6291966
  2. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  3. Apolipoprotein AIMarburg: studies on two kindreds with a mutant of human apolipoprotein AI.
    Hum Genet. 1982;61(4):329-37 PMID: 6818131
  4. Genetic fine-structure mapping in human chromosome 11 by use of repetitive DNA sequences.
    Proc Natl Acad Sci U S A. 1982 Dec;79(24):7804-8 PMID: 6961451
  5. Assignment of the structural gene coding for albumin to human chromosome 4.
    Hum Genet. 1982;62(4):337-41 PMID: 7166310
  6. Genetic control of lipid transport in mice. II. Genes controlling structure of high density lipoproteins.
    J Biol Chem. 1983 Apr 25;258(8):5071-8 PMID: 6403543
  7. Resistance of Chinese hamster ovary cell chromatin to endonuclease digestion. I. Reversal by cAMP.
    Exp Cell Res. 1983 Apr 15;145(1):57-62 PMID: 6303820
  8. Nucleotide sequence of cloned cDNA of human apolipoprotein A-I.
    Nucleic Acids Res. 1983 Jun 11;11(11):3703-15 PMID: 6304641
  9. Gene structure of human apolipoprotein A1.
    Nucleic Acids Res. 1983 May 11;11(9):2827-37 PMID: 6406984
  10. Assignment of the alpha 1-antitrypsin gene and a sequence-related gene to human chromosome 14 by molecular hybridization.
    Am J Hum Genet. 1983 May;35(3):385-92 PMID: 6602546
  11. Linkage of human apolipoproteins A-I and C-III genes.
    Nature. 1983 Jul 28-Aug 3;304(5924):371-3 PMID: 6308458
  12. Protein-lipid relationships in human plasma. II. In atherosclerosis and related conditions.
    Am J Med. 1951 Oct;11(4):480-93 PMID: 14885223
  13. Genetics of somatic mammalian cells: genetic, immunologic, and biochemical analysis with Chinese hamster cell hybrids containing selected human chromosomes.
    Proc Natl Acad Sci U S A. 1976 Jan;73(1):193-7 PMID: 1061117
  14. Relationships between plasma lipoprotein cholesterol concentrations and the pool size and metabolism of cholesterol in man.
    Atherosclerosis. 1976 May-Jun;23(3):535-47 PMID: 178328
  15. Cholesterol content and sterol synthesis in human skin fibroblasts and rat aortic smooth muscle cells exposed to lipoprotein-depleted serum and high density apolipoprotein/phospholipid mixtures.
    Biochim Biophys Acta. 1976 May 27;431(2):347-58 PMID: 181057
  16. Post delivery care of the edentulous geriatric patient.
    Bull Phila Cty Dent Soc. 1975 Summer;40(8):16-9 PMID: 1065414
  17. High density lipoprotein as a protective factor against coronary heart disease. The Framingham Study.
    Am J Med. 1977 May;62(5):707-14 PMID: 193398
  18. The intestine as a source of apolipoprotein A1.
    Proc Natl Acad Sci U S A. 1977 Jun;74(6):2569-73 PMID: 196292
  19. Genetics of cell-surface antigens: regional mapping of three components of the human cell-surface antigen complex, AL, on chromosome 11.
    Somatic Cell Genet. 1977 Jul;3(4):421-9 PMID: 601680
  20. The amino acid sequence of human APOA-I, an apolipoprotein isolated from high density lipoproteins.
    Biochem Biophys Res Commun. 1978 Feb 14;80(3):623-30 PMID: 204308
  21. Regional mapping of the gene for human lysosomal acid phosphatase (ACP2) using a hybrid clone panel containing segments of human chromosome 11.
    Hum Genet. 1978 Nov 24;45(1):1-10 PMID: 730175
  22. Relative contributions by liver and intestine to individual plasma apolipoproteins in the rat.
    J Biol Chem. 1979 Aug 10;254(15):7316-22 PMID: 457683
  23. Precise localization of human beta-globin gene complex on chromosome 11.
    Proc Natl Acad Sci U S A. 1979 Oct;76(10):5239-42 PMID: 291941
  24. Molecular cloning of the gene sequences of a major apoprotein in avian very low density lipoproteins.
    Biochemistry. 1980 Nov 25;19(24):5631-7 PMID: 6257284
  25. Characterization, cell-free synthesis, and processing of apolipoprotein A-I of rat high-density lipoproteins.
    Biochemistry. 1981 Apr 28;20(9):2470-5 PMID: 6786335
  26. Regional gene assignment of human porphobilinogen deaminase and esterase A4 to chromosome 11q23 leads to 11qter.
    Proc Natl Acad Sci U S A. 1981 Sep;78(9):5734-8 PMID: 6946513
  27. The primary translation product of rat intestinal apolipoprotein A-I mRNA is an unusual preproprotein.
    J Biol Chem. 1982 Jan 25;257(2):971-8 PMID: 6798036
  28. Isolation and chromosomal localization of unique DNA sequences from a human genomic library.
    Proc Natl Acad Sci U S A. 1982 Feb;79(3):865-9 PMID: 7038688
  29. Abnormal apoprotein A-I isoprotein composition in patients with Tangier disease.
    J Biol Chem. 1982 May 10;257(9):4978-86 PMID: 7068673
  30. Intestinal Biosynthesis of apolipoproteins in the rat: apoE and apoA-I mRNA translation and regulation.
    Metabolism. 1982 Sep;31(9):861-5 PMID: 6811833
  31. Isolation of the human HDL apoprotein A1 gene.
    Nucleic Acids Res. 1982 Aug 25;10(16):4873-82 PMID: 6290991
  32. Ischemic heart disease, atherosclerosis, and longevity.
    Circulation. 1966 Oct;34(4):679-97 PMID: 5921763
  33. A membrane-filter technique for the detection of complementary DNA.
    Biochem Biophys Res Commun. 1966 Jun 13;23(5):641-6 PMID: 5963888
  34. A protein cofactor of lecithin:cholesterol acyltransferase.
    Biochem Biophys Res Commun. 1972 Feb 25;46(4):1493-8 PMID: 4335615
  35. Staining of some specific regions of human chromosomes, particularly the secondary constriction of No. 9.
    Nat New Biol. 1972 Jul 26;238(82):122-4 PMID: 4114702
  36. Lecithin: cholesterol acyltransferase: effects of substrate composition upon enzyme activity.
    Biochim Biophys Acta. 1972 Aug 11;270(4):513-8 PMID: 4340992
  37. The metabolic role of lecithin: cholesterol acyltransferase: perspectives form pathology.
    Adv Lipid Res. 1973;11:1-65 PMID: 4354500
  38. Identification of chick chromosomes in cell hybrids formed between chick erythrocytes and adenine-requiring mutants of Chinese hamster cells.
    Proc Natl Acad Sci U S A. 1973 Oct;70(10):2893-8 PMID: 4517942
  39. The removal of cholesterol from Landschütz ascites cells by high-density apolipoprotein.
    Biochim Biophys Acta. 1973 Nov 29;326(2):232-44 PMID: 4128802
  40. Regulation of cellular sterol flux and synthesis by human serum lipoproteins.
    Biochim Biophys Acta. 1974 Jul 26;360(1):38-55 PMID: 4369129
  41. A comparative study on the removal of cellular lipids from Landschütz ascites cells by human plasma apolipoproteins.
    J Biol Chem. 1975 Sep 25;250(18):7204-9 PMID: 170257
  42. Isolation and characterization of cDNA clones for human apolipoprotein A-I.
    Proc Natl Acad Sci U S A. 1982 Nov;79(22):6861-5 PMID: 6294659
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.81.2.508
Published
1984-01-00
Pages
508-11
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC344707
Subset
IM
Grants
NCI NIH HHS · CA-18734 · United States
NICHD NIH HHS · HD-02080 · United States
NHLBI NIH HHS · HL-27341 · United States
External Links
PubMed source DOI: 10.1073/pnas.81.2.508 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2