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PMID: 6429051 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Purification and characterization of Staphylococcus aureus type 8 capsular polysaccharide.

Infection and immunity ·Vol. 45 ·No. 1 ·1984-07-00 ·Pages 87-93

Fournier JM, Vann WF, Karakawa WW

Abstract

Clinical isolates of Staphylococcus aureus have been previously classified into eight types on the basis of their capsular polysaccharide. The high prevalence of the type 8 capsular polysaccharide among bacteremic isolates suggests the importance of this capsular antigen in staphylococcal disease. The capsular polysaccharide was purified from extracts of three clinical isolates of S. aureus type 8 of different geographic and temperal origin by ion-exchange chromatography and gel filtration. Gas chromatography, gas chromatography-mass spectrometry, and 13C-nuclear magnetic resonance showed that the type 8 capsular polysaccharide is composed of O-acetyl groups, N-acetylfucosamine, and an aminouronic acid similar to N-acetylgalactosaminouronic acid. The purified polysaccharide reacted only with type 8 antiserum in double diffusion experiments. Our analysis shows that the type 8 polysaccharide is both chemically and serologically distinct from teichoic acid and previously characterized polysaccharides of S. aureus.

MeSH Terms
Carbohydrates/analysis Chromatography, Gas Chromatography, Gel Chromatography, Ion Exchange Humans Immunodiffusion Immunoelectrophoresis Magnetic Resonance Spectroscopy Polysaccharides, Bacterial/isolation & purification Staphylococcus aureus/immunology,isolation & purification
Chemicals
Carbohydrates Polysaccharides, Bacterial
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Fournier J M
Vann W F
Karakawa W W
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35 references, click to expand
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.45.1.87-93.1984
Published
1984-07-00
Pages
87-93
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC263273
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.45.1.87-93.1984 PMC Full Text View journal details
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