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PMID: 6433 Published · ppublish English Journal Article

Characterization of group B colicin-resistant mutants of Escherichia coli K-12: colicin resistance and the role of enterochelin.

Journal of bacteriology ·Vol. 127 ·No. 1 ·1976-07-00 ·Pages 218-28

Pugsley AP, Reeves P

Abstract

Nine classes of group B colicin-resistant mutants were examined to study the role of enterochelin in colicin resistance. Four of the mutants studied (cbt, exbC, exbB, and tonB) hypersecreted enterochelin. Enterochelin hypersecretion was apparently responsible for resistance of the exbC mutant to colicins G and H and for resistance of the exbB mutant to colicins G, H, Ia, Ib, S1, and V. All four mutants scored as colicin B tolerant, even in the absence of enterochelin synthesis. The mutants produced substantially increased amounts of two high-molecular-weight outer membrane polypeptides when grown under limiting iron conditions. The presence of these polypeptides was correlated with increased colicin B-neutralizing activity in the outer membrane preparations.

MeSH Terms
2,2'-Dipyridyl/pharmacology Bacterial Proteins/biosynthesis Cell Membrane/metabolism Citrates/pharmacology Colicins/pharmacology Deferoxamine/pharmacology Drug Resistance, Microbial Edetic Acid/pharmacology Enterobactin/metabolism,pharmacology Escherichia coli/drug effects,metabolism Genes Molecular Weight Mutation Peptide Biosynthesis Serine/analogs & derivatives
Chemicals
Bacterial Proteins Citrates Colicins Enterobactin Serine 2,2'-Dipyridyl Edetic Acid Deferoxamine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Pugsley A P
Reeves P
References (28)
28 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.127.1.218-228.1976
Published
1976-07-00
Pages
218-28
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC233054
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.127.1.218-228.1976 PMC Full Text View journal details
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