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PMID: 6433345 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

5' untranslated sequences are required for the translational control of a yeast regulatory gene.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 81 ·No. 16 ·1984-08-00 ·Pages 5096-100

Thireos G, Penn MD, Greer H

Abstract

In yeast, many genes encoding amino acid biosynthetic enzymes are subject to a common regulatory system called the general control of amino acid biosynthesis. The product of the regulatory gene GCN4 is required for an increase in transcription of general control-regulated genes when yeast are grown under amino acid-starvation conditions. In this report, we show that the expression of the GCN4 gene is regulated at the translational level: the efficiency of translation of the GCN4 mRNA is dramatically increased during growth under amino acid-starvation conditions. The complete nucleotide sequence of the GCN4 gene, presented here, reveals the existence of an unusually long 5' untranslated region in the corresponding mRNA. In vivo analysis of the effects of a deletion in this 5' leader has enabled us to define a region required for the translational regulation of the GCN4 mRNA.

MeSH Terms
Amino Acid Sequence Base Sequence Genes Genes, Fungal Genes, Regulator Nucleic Acid Hybridization Plasmids Protein Biosynthesis Saccharomyces cerevisiae/genetics beta-Galactosidase/genetics
Chemicals
beta-Galactosidase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Thireos G
Penn M D
Greer H
References (32)
32 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.81.16.5096
Published
1984-08-00
Pages
5096-100
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC391644
Subset
IM
Grants
NIGMS NIH HHS · GM25207 · United States
Databases
GENBANK
K02649
External Links
PubMed source DOI: 10.1073/pnas.81.16.5096 PMC Full Text View journal details
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