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PMID: 6433849 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Isolation and characterization of mutant Pseudomonas aeruginosa strains unable to assimilate nitrate.

Archives of microbiology ·Vol. 138 ·No. 2 ·1984-06-00 ·Pages 124-30

Jeter RM, Ingraham JL

Abstract

Single-site mutants of Pseudomonas aeruginosa that lack the ability aerobically to assimilate nitrate and nitrite as sole sources of nitrogen have been isolated. Twenty-one of these have been subdivided into four groups by transductional analysis. Mutants in only one group, designated nis, lost assimilatory nitrite reductase activity. Mutants in the other three transductional groups, designated ntmA, ntmB, ntmC, display a pleiotropic phenotype: utilization of a number of nitrogen-containing compounds including nitrite as sole nitrogen sources is impaired. Assimilatory nitrite reductase was shown to be the major route by which hydroxylamine is reduced in aerobically-grown cells.

MeSH Terms
Gene Expression Regulation Genes Genes, Bacterial Mutation NADH, NADPH Oxidoreductases/genetics Nitrates/metabolism Nitrite Reductases/genetics,metabolism Nitrites/metabolism Nitrogen/metabolism Phenotype Pseudomonas aeruginosa/enzymology,genetics,isolation & purification Transduction, Genetic
Chemicals
Nitrates Nitrites NADH, NADPH Oxidoreductases Nitrite Reductases Nitrogen
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Jeter R M
Ingraham J L
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Article Info
Journal
Archives of microbiology
Abbr.
Arch Microbiol
ISSN
0302-8933
DOI
10.1007/BF00413012
Published
1984-06-00
Pages
124-30
Language
English
Region
Germany
NLM ID
0410427
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00413012 View journal details
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