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PMID: 6434549 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Intermediate-sized filaments in Drosophila tissue culture cells.

The Journal of cell biology ·Vol. 99 ·No. 4 Pt 1 ·1984-10-00 ·Pages 1468-77

Walter MF, Biessmann H

Abstract

In using a monoclonal antibody against a major cytoplasmic protein of 46,000 mol wt, we have characterized an intermediate-sized (10 nm) filamentous cytoskeleton in Drosophila melanogaster tissue culture cells. Indirect immunofluorescence, immunoelectron microscopy, and protein blotting show that this cytoskeleton exhibits features typical of the vertebrate vimentin cytoskeleton, including the diameter and appearance of filaments, sensitivity to 10(-6) M colcemid, and insolubility in buffers containing 1% Triton X-100. The antibody cross-reacts with vimentin and desmin from baby hamster kidney cells and stains a vimentin cytoskeleton in the vertebrate Chinese hamster ovary cell line. We, therefore, conclude that the 46,000-mol wt Drosophila protein is homologous to vertebrate vimentin. Three minor, higher-molecular-weight polypeptides are also detected in the Drosophila cells that react with the antibody. At least two of these are members of a family of proteins with properties resembling those of the 46,000-mol wt intermediate filament protein.

MeSH Terms
Animals Antibodies, Monoclonal Cells, Cultured Cytoskeleton/ultrastructure Drosophila melanogaster/ultrastructure Electrophoresis, Polyacrylamide Gel Fluorescent Antibody Technique Intermediate Filament Proteins/analysis Microscopy, Electron Molecular Weight Peptide Fragments/analysis
Chemicals
Antibodies, Monoclonal Intermediate Filament Proteins Peptide Fragments
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Walter M F
Biessmann H
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70 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.99.4.1468
Published
1984-10-00
Pages
1468-77
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2113308
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.99.4.1468 PMC Full Text View journal details
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