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PMID: 6435513 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Correlation between lipopolysaccharide structure and permeability resistance in beta-lactam-resistant Pseudomonas aeruginosa.

Antimicrobial agents and chemotherapy ·Vol. 26 ·No. 2 ·1984-08-00 ·Pages 181-6

Godfrey AJ, Hatlelid L, Bryan LE

Abstract

Four beta-lactam-resistant permeability mutants of Pseudomonas aeruginosa PAO503 were studied. The resistance phenotypes were correlated to changes within the lipopolysaccharide. Two of the mutants, PCC1 and PCC19, were shown to differentiate between beta-lactams on the basis of relative hydrophobicity. The more hydrophilic antibiotics were less effective at inhibiting these strains. This phenotype was correlated to the presence of mannose, in measurable quantities, in lipopolysaccharide isolated from these strains. The other two strains, PCC23 and PCC100, differentiated between cephem antibiotics on the basis of electrical charge. The presence of a positive charge markedly increased the relative efficiency of an antibiotic. This correlation did not hold for penam derivatives, with the lower-molecular-weight, dianionic molecules being the most effective. Mutants of this type were changed in the amount of "side chain" sugars or, to minor extent, in their outer membrane protein profiles.

MeSH Terms
Amino Acids/analysis Amino Sugars/analysis Anti-Bacterial Agents/metabolism,pharmacology Bacterial Outer Membrane Proteins/metabolism Bacterial Proteins/metabolism Carrier Proteins/analysis Cell Membrane Permeability Drug Resistance, Microbial Electrophoresis, Polyacrylamide Gel Hexosyltransferases Lipopolysaccharides/analysis Muramoylpentapeptide Carboxypeptidase/analysis Penicillin-Binding Proteins Peptidyl Transferases Pseudomonas aeruginosa/drug effects,metabolism beta-Lactams
Chemicals
Amino Acids Amino Sugars Anti-Bacterial Agents Bacterial Outer Membrane Proteins Bacterial Proteins Carrier Proteins Lipopolysaccharides Penicillin-Binding Proteins beta-Lactams Peptidyl Transferases Hexosyltransferases Muramoylpentapeptide Carboxypeptidase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Godfrey A J
Hatlelid L
Bryan L E
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Article Info
Journal
Antimicrobial agents and chemotherapy
Abbr.
Antimicrob Agents Chemother
ISSN
0066-4804
DOI
10.1128/AAC.26.2.181
Published
1984-08-00
Pages
181-6
Language
English
Region
United States
NLM ID
0315061
PMCID
PMC284115
Subset
IM
External Links
PubMed source DOI: 10.1128/AAC.26.2.181 PMC Full Text View journal details
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