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PMID: 6803665 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Mutation of Pseudomonas aeruginosa specifying reduced affinity for penicillin G.

Antimicrobial agents and chemotherapy ·Vol. 21 ·No. 2 ·1982-02-00 ·Pages 216-23

Godfrey AJ, Bryan LE

Abstract

A mutant of Pseudomonas aeruginosa strain PAO503 was isolated after ethane-methane-sulfonate mutagenesis and selection of ticarcillin. The mutant, PCC17, displayed reduced affinity for [14C] penicillin G at all of its penicillin-binding proteins as well as a general increase in resistance to all the beta-lactam antibiotics tested. The mutation designated pbpA has been mapped by FP-2-mediated conjugation and was located distal to the proA locus and 33% linked to it. The two loci were not cotransducible with phage F116L. PCC17 and exconjugants produced from it had similar phenotypes, displayed the reduced affinity for [14C] penicillin G, had similar resistance profiles, and had an increased amount of protein corresponding to penicillin-binding protein 6. On back mutation the pbpA locus reverted to the PAO503 phenotype.

MeSH Terms
Anti-Bacterial Agents/pharmacology Carrier Proteins/metabolism Cell Membrane Permeability Lactams/pharmacology Mutagenicity Tests Mutation Penicillin G/metabolism Penicillin Resistance Phenotype Pseudomonas aeruginosa/drug effects,genetics,metabolism
Chemicals
Anti-Bacterial Agents Carrier Proteins Lactams Penicillin G
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Godfrey A J
Bryan L E
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23 references, click to expand
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Article Info
Journal
Antimicrobial agents and chemotherapy
Abbr.
Antimicrob Agents Chemother
ISSN
0066-4804
DOI
10.1128/AAC.21.2.216
Published
1982-02-00
Pages
216-23
Language
English
Region
United States
NLM ID
0315061
PMCID
PMC181862
Subset
IM
External Links
PubMed source DOI: 10.1128/AAC.21.2.216 PMC Full Text View journal details
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