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PMID: 6436686 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Sequences responsible for transcription termination on a gene segment in Saccharomyces cerevisiae.

Molecular and cellular biology ·Vol. 4 ·No. 8 ·1984-08-00 ·Pages 1515-20

Henikoff S, Cohen EH

Abstract

We have mapped a signal sequence for mRNA 3'-end formation in Saccharomyces cerevisiae by using a Drosophila melanogaster DNA segment that complements a yeast adenine-8 mutation. That the 3' end of the transcript in S. cerevisiae nearly coincides with that in D. melanogaster is consistent with the possibility that mRNA termini are similarly determined in both organisms. Deletion analysis reveals that the complete signal is no more than 21 base pairs long. Part of the signal is the sequence TTTTTATA, which is seen in the termination region of several yeast genes. TTTTTATA appears to be able to act autonomously as a partial termination signal. The efficiency of the complete signal is affected by substitution of sequences downstream from it. This modulation of the effect of a signal is consistent with termination in S. cerevisiae, resembling rho-dependent termination in bacteria.

MeSH Terms
Animals Base Sequence Chromosome Deletion DNA/genetics Drosophila melanogaster/genetics Genes, Fungal Mutation Nucleic Acid Hybridization Plasmids RNA, Messenger/genetics Saccharomyces cerevisiae/genetics Transcription, Genetic
Chemicals
RNA, Messenger DNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Henikoff S
Cohen E H
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29 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.4.8.1515-1520.1984
Published
1984-08-00
Pages
1515-20
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC368942
Subset
IM
Grants
NIGMS NIH HHS · GM29009 · United States
Databases
GENBANK
K01946
External Links
PubMed source DOI: 10.1128/mcb.4.8.1515-1520.1984 PMC Full Text View journal details
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