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PMID: 6438630 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Sequence of the bacteriophage SP01 gene coding for transcription factor 1, a viral homologue of the bacterial type II DNA-binding proteins.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 81 ·No. 22 ·1984-11-00 ·Pages 7031-5

Greene JR, Brennan SM, Andrew DJ, Thompson CC, Richards SH, Heinrikson RL, Geiduschek EP

Abstract

The Bacillus subtilis phage SP01, whose DNA contains 5-hydroxymethyluracil (hmUra) in place of thymine, codes for an abundant, small, basic protein called TF1. TF1 binds preferentially to hydroxymethyluracil-containing DNA and thereby selectively inhibits transcription of such DNA in vitro. The gene for TF1 has been sequenced. We find that this viral protein is a homologue of the ubiquitous bacterial type II DNA-binding proteins. The three-dimensional structure of one of these bacterial proteins has recently been determined. We are able to discern common as well as distinctive features in the amino acid sequence and the three-dimensional structure of the homologous viral protein.

MeSH Terms
Amino Acid Sequence Bacillus subtilis/genetics Bacteriophages/genetics Base Sequence DNA-Binding Proteins/genetics Genes, Viral Structure-Activity Relationship Transcription Factors/genetics
Chemicals
DNA-Binding Proteins Transcription Factors
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Greene J R
Brennan S M
Andrew D J
Thompson C C
Richards S H
Heinrikson R L
Geiduschek E P
References (28)
28 references, click to expand
  1. A general method applicable to the search for similarities in the amino acid sequence of two proteins.
    J Mol Biol. 1970 Mar;48(3):443-53 PMID: 5420325
  2. Quantitative procedures for use with the Edman-Begg sequenator. Partial sequences of two unusual immunoglobulin light chains, Rzf and Sac.
    Biochemistry. 1971 Dec 21;10(26):4912-21 PMID: 5134536
  3. Purification of the bacteriophage SP01 transcription factor 1.
    J Biol Chem. 1972 Jun 10;247(11):3571-8 PMID: 4624121
  4. Specificity of the weak binding between the phage SPO1 transcription-inhibitory protein, TF1, and SPO1 DNA.
    Biochemistry. 1977 Apr 5;16(7):1473-85 PMID: 402939
  5. Sequence data handling by computer.
    Nucleic Acids Res. 1977 Nov;4(11):4037-51 PMID: 593900
  6. Effects of the transciption inhibitory protein, TF1, on phage SP01 promoter complex formation and stability.
    J Mol Biol. 1977 Dec 25;117(4):825-42 PMID: 415142
  7. EcoRI cleavage of DNA from Bacillus subtilis phage SPO1.
    Virology. 1978 Apr;85(2):601-5 PMID: 96586
  8. Localization of the HU protein on the Escherichia coli nucleoid.
    Cold Spring Harb Symp Quant Biol. 1978;42 Pt 1:439-47 PMID: 209942
  9. The role of tyrosine in the association of proteins and nucleic acids. Specific recognition of single-stranded nucleic acids by tyrosine-containing peptides.
    J Biol Chem. 1979 Jan 10;254(1):75-82 PMID: 758326
  10. E. coli DNA binding protein HU forms nucleosomelike structure with circular double-stranded DNA.
    Cell. 1979 Jun;17(2):265-74 PMID: 222478
  11. Restriction cleavage map of SP01 DNA: general location of early, middle, and late genes.
    J Virol. 1979 Jul;31(1):156-71 PMID: 116010
  12. Regulatory sequences involved in the promotion and termination of RNA transcription.
    Annu Rev Genet. 1979;13:319-53 PMID: 94251
  13. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  14. Solid-phase synthesis of polynucleotides. III. Synthesis of polynucleotides with defined sequences by the block coupling phosphotriester method.
    Nucleic Acids Res. 1980 Nov 25;8(22):5491-505 PMID: 7465419
  15. The use of synthetic oligonucleotides as hybridization probes. II. Hybridization of oligonucleotides of mixed sequence to rabbit beta-globin DNA.
    Nucleic Acids Res. 1981 Feb 25;9(4):879-94 PMID: 7232206
  16. A transcriptional map of the bacteriophage SPO1 genome. III. A region of early and middle promoters (the gene 28 region).
    Virology. 1981 Jul 30;112(2):572-88 PMID: 6266141
  17. The reaction of aromatic peptides with double helical DNA. Quantitative characterisation of a two step reaction scheme.
    Biophys Chem. 1981 Aug;13(4):283-90 PMID: 6793107
  18. Purification and characterisation of a small DNA-binding protein, HB, from Bacillus globigii.
    Eur J Biochem. 1982 Mar 1;122(3):627-32 PMID: 7037410
  19. On the DNA binding protein II from Bacillus stearothermophilus. II. The amino acid sequence and its relation to those of homologous proteins from other prokaryotes.
    J Biol Chem. 1983 Mar 25;258(6):4007-11 PMID: 6300069
  20. Base sequence and helix structure variation in B and A DNA.
    J Mol Biol. 1983 May 25;166(3):419-41 PMID: 6854650
  21. Establishing homologies in protein sequences.
    Methods Enzymol. 1983;91:524-45 PMID: 6855599
  22. Proteins from the prokaryotic nucleoid. High-resolution 1H NMR spectroscopic study of Escherichia coli DNA-binding proteins NS1 and NS2.
    Eur J Biochem. 1984 Jan 2;138(1):193-200 PMID: 6363068
  23. Protein HU in the enzymatic replication of the chromosomal origin of Escherichia coli.
    Proc Natl Acad Sci U S A. 1984 Jan;81(2):424-8 PMID: 6364143
  24. Interaction of a tryptophan-containing peptide with chromatin core particles. A fluorescence study.
    FEBS Lett. 1984 Apr 24;169(2):205-10 PMID: 6714425
  25. 3-A resolution structure of a protein with histone-like properties in prokaryotes.
    Nature. 1984 Aug 2-8;310(5976):376-81 PMID: 6540370
  26. A system to study promoter and terminator signals recognized by Escherichia coli RNA polymerase.
    Gene Amplif Anal. 1981;2:383-415 PMID: 6101056
  27. THE POSSIBLE ROLE OF RECOMBINATION IN THE INFECTION OF COMPETENT BACILLUS SUBTILIS BY BACTERIOPHAGE DEOXYRIBONUCLEIC ACID.
    Virology. 1964 Dec;24:552-62 PMID: 14240402
  28. Analysis of amino acid phenylthiohydantoins by gas chromatography.
    J Biol Chem. 1969 Oct 25;244(20):5597-607 PMID: 5348603
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.81.22.7031
Published
1984-11-00
Pages
7031-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC392070
Subset
IM
Databases
GENBANK
K02381
External Links
PubMed source DOI: 10.1073/pnas.81.22.7031 PMC Full Text View journal details
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