N,N'-dicyclohexylcarbodiimide binds specifically to a single glutamyl residue of the proteolipid subunit of the mitochondrial adenosinetriphosphatases from Neurospora crassa and Saccharomyces cerevisiae.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 77 ·No. 2 ·1980-02-00 ·Pages 785-9

Sebald W, Machleidt W, Wachter E

Abstract

The N,N'-dicyclohexylcarbodiimide-binding proteolipid subunit of the mitochondrial adenosinetriphosphatases (ATP phosphohydrolase, EC 3.6.1.3) of Neurospora crassa and Saccharomyces cerevisiae were purified from mitochondria incubated with the radioactively labeled inhibitor. The specifically labeled subunit was cleaved with cyanogen bromide and N-bromosuccinimide, and the resultant fragments were separated by gel chromatography in the presence of 80% (vol/vol) formic acid. The N,N'-dicyclohexylcarbodiimide label was recovered in each organism exclusively in a 17-residue fragment. Further analysis by automated solid-phase Edman degradation revealed that the bound label was present at only one position, corresponding to a glutamyl residue. The N,N'-dicyclohexylcarbodiimide-modified glutamyl residue is the only identical acidic position in both proteins and occurs in the middle of a hydrophobic sequence of about 25 residues.

MeSH Terms

Adenosine Triphosphatases/antagonists & inhibitors Amino Acid Sequence Binding Sites Carbodiimides/pharmacology Dicyclohexylcarbodiimide/metabolism,pharmacology Glutamates/metabolism Mitochondria/enzymology Neurospora crassa/enzymology Peptide Fragments Proteolipids/metabolism Saccharomyces cerevisiae/enzymology

Chemicals

Carbodiimides Glutamates Peptide Fragments Proteolipids Dicyclohexylcarbodiimide Adenosine Triphosphatases

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Sebald W

Machleidt W

Wachter E

References (25)

25 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.77.2.785

Published

1980-02-00

Pages

785-9

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC348365

Subset

External Links

PubMed source DOI: 10.1073/pnas.77.2.785 PMC Full Text View journal details

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N,N'-dicyclohexylcarbodiimide binds specifically to a single glutamyl residue of the proteolipid subunit of the mitochondrial adenosinetriphosphatases from Neurospora crassa and Saccharomyces cerevisiae.

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