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PMID: 6444726 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Plasminogen activator of islets of Langerhans: modulation by glucose and correlation with insulin production.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 77 ·No. 2 ·1980-02-00 ·Pages 875-9

Virji MA, Vassalli JD, Estensen RD, Reich E

Abstract

Islets of Langerhans isolated from rat pancreas contain and secrete plasminogen activator (PA). Production of PA is increased up to 15-fold by culture in the presence of high concentrations of glucose, and the dose-response curves for the effect of glucose on secretion of PA and on insulin are superimposable. Alloxan, a diabetogenic agent that is selectively cytotoxic for beta cells, abolishes the PA response to glucose. Various agents and hormones that affect beta-cell function affect the secretion of PA in a manner parallel to their modulation of insulin secretion. These observations suggest that PA is produced by beta cells and that enzyme synthesis and secretion are regulated in concert with those of the hormone. The potential role of PA and of plasmin in the physiology of the islets is considered. In particular, because plasmin cleaves proinsulin to a product that is electrophoretically indistinguishable from insulin, it is suggested that the PA/plasmin system may play a part in the conversion of proinsulin to the active hormone.

MeSH Terms
Alloxan/pharmacology Animals Cells, Cultured Dose-Response Relationship, Drug Fibrinolysin/metabolism Glucose/pharmacology Insulin/metabolism Insulin Secretion Islets of Langerhans/drug effects,metabolism Male Molecular Weight Plasminogen Activators/biosynthesis Proinsulin/metabolism Rats
Chemicals
Insulin Alloxan Proinsulin Plasminogen Activators Fibrinolysin Glucose
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Virji M A
Vassalli J D
Estensen R D
Reich E
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29 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.77.2.875
Published
1980-02-00
Pages
875-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC348384
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.77.2.875 PMC Full Text View journal details
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