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PMID: 6453132 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Charge of circulating immune complexes as a factor in glomerular basement membrane localization in mice.

The Journal of clinical investigation ·Vol. 67 ·No. 5 ·1981-05-00 ·Pages 1305-13

Gallo GR, Caulin-Glaser T, Lamm ME

Abstract

The effect of the charge of circulating immune complexes on glomerular localization was studied in a model of passive serum sickness. Preformed immune complexes of heterogeneous or restricted charge, prepared in vitro from isoelectrically focused or chemically modified proteins, were injected intravenously into mice. The distribution of immune complexes in the kidney was compared by immunofluorescence and electron microscopy. Cationic but not anionic or electrophoretically heterogeneous immune complexes gave rise to diffuse localization in the glomerular basement membrane. The binding in subepithelial and subendothelial sites correlated with the known distribution of structural anionic sites. The observations suggest that electrostatic interactions between fixed anionic sites and immune complexes may be an important factor in glomerular trapping. Alternative mechanisms based on initial localization of excess free cationic antigen cannot be completely excluded and are also considered.

MeSH Terms
Animals Antigen-Antibody Complex Basement Membrane/immunology Disease Models, Animal Immune Complex Diseases/physiopathology Isoelectric Point Kidney Glomerulus/immunology,ultrastructure Mice Microscopy, Electron
Chemicals
Antigen-Antibody Complex
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Gallo G R
Caulin-Glaser T
Lamm M E
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31 references, click to expand
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Article Info
Journal
The Journal of clinical investigation
Abbr.
J Clin Invest
ISSN
0021-9738
DOI
10.1172/jci110159
Published
1981-05-00
Pages
1305-13
Language
English
Region
United States
NLM ID
7802877
PMCID
PMC370697
Subset
IM
Grants
NIAID NIH HHS · AI-15071 · United States
NCI NIH HHS · CA-23885 · United States
External Links
PubMed source DOI: 10.1172/jci110159 PMC Full Text View journal details
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