Antibodies to membrane structures that distinguish suppressor/cytotoxic and helper T lymphocyte subpopulations block the mixed leukocyte reaction in man.
Two major subsets of human T lymphocytes that are functionally analogous to the mouse Lyt-2+ and Lyt-2- subsets have been defined by their expression of two thymus-dependent membrane antigens, Leu-2 and Leu-3. Leu-2+,3- cells have suppressor/cytotoxic functions and Leu-2-,3+ cells have helper functions. These studies were designed to determine the effects of adding IgG1 monoclonal anti-Leu-2 and anti-Leu-3 antibodies to the mixed leukocyte reaction (MLR). At high concentrations, each antibody partially inhibited the proliferative response of unseparated T cells and abolished the response of the isolated subset having the appropriate phenotype. An IgG1 monoclonal antibody to HLA-A2 and an IgG2a antibody to Leu-1, a pan-T antigen, failed to inhibited the MLR. These results suggest that the Leu-2 and Leu-3 antigens may have a direct role in the mechanism whereby T cells recognize and respond to alloantigen.
19 references, click to expand
-
Cytochemical identification of monocytes and granulocytes.Am J Clin Pathol. 1971 Mar;55(3):283-90 PMID: 5549896
-
Genetic correlation of a mouse light chain variable region marker with a thymocyte surface antigen.J Exp Med. 1974 Nov 1;140(5):1432-7 PMID: 4547628
-
Expression of T-cell differentiation antigens on effector cells in cell-mediated cytotoxicity in vitro. Evidence for functional heterogeneity related to the surface phenotype of T cells.J Exp Med. 1975 Jan 1;141(1):227-41 PMID: 1078839
-
Functional subclasses of T lymphocytes bearing different Ly antigens. II. Cooperation between subclasses of Ly+ cells in the generation of killer activity.J Exp Med. 1975 Jun 1;141(6):1390-9 PMID: 1092799
-
Antibody-dependent cellular cytotoxicity by allosensitized human T cells.J Exp Med. 1978 Feb 1;147(2):605-10 PMID: 146728
-
Two functionally distinct subpopulations of human T cells that collaborate in the generation of cytotoxic cells responsible for cell-mediated lympholysis.J Immunol. 1978 Apr;120(4):1423-8 PMID: 147908
-
"Panning" for lymphocytes: a method for cell selection.Proc Natl Acad Sci U S A. 1978 Jun;75(6):2844-8 PMID: 351618
-
Evidence for close linkage of a mouse light chain marker with the Ly-2,3 locus.J Immunol. 1978 Oct;121(4):1585-90 PMID: 100557
-
Monoclonal antibody to a human histocompatibility alloantigen, HLA-A2.Nature. 1978 Nov 23;276(5686):397-9 PMID: 714164
-
Cytotoxic T cells: Lyt phenotype and blocking of killing activity by Lyt antisera.Proc Natl Acad Sci U S A. 1979 Apr;76(4):1977-81 PMID: 88050
-
Mouse alloantibodies capable of blocking cytotoxic T-cell function. I. Relationship between the antigen reactive with blocking antibodies and the Lyt-2 locus.J Exp Med. 1979 Sep 19;150(3):432-44 PMID: 113478
-
Separation of functional subsets of human T cells by a monoclonal antibody.Proc Natl Acad Sci U S A. 1979 Aug;76(8):4061-5 PMID: 315070
-
Alloantigen-induced T-cell proliferation: Lyt phenotype of responding cells and blocking of proliferation by Lyt antisera.Proc Natl Acad Sci U S A. 1980 May;77(5):2890-4 PMID: 6967215
-
Biochemical analysis of human T lymphocyte differentiation antigens T4 and T5.Science. 1980 Jul 25;209(4455):520-1 PMID: 6967228
-
Blocking effect of lyt-2 antibodies on T cell functions.J Exp Med. 1980 Sep 1;152(3):674-87 PMID: 6967947
-
IgG or IgM monoclonal antibodies reactive with different determinants on the molecular complex bearing Lyt 2 antigen block T cell-mediated cytolysis in the absence of complement.J Immunol. 1980 Dec;125(6):2665-72 PMID: 6159417
-
Studies of a human T lymphocyte antigen recognized by a monoclonal antibody.Proc Natl Acad Sci U S A. 1981 Mar;78(3):1791-5 PMID: 7015346
-
Evolutionary conservation of surface molecules that distinguish T lymphocyte helper/inducer and cytotoxic/suppressor subpopulations in mouse and man.J Exp Med. 1981 Feb 1;153(2):310-23 PMID: 6165796
-
Thymus-dependent membrane antigens in man: inhibition of cell-mediated lympholysis by monoclonal antibodies to TH2 antigen.Proc Natl Acad Sci U S A. 1981 Jan;78(1):544-8 PMID: 6787593
Contact
No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong
Qilu Normal University · Genelibs Bioinformatics Lab
750 Shunhua Rd, Jinan
2F, Bldg F, University Science Park
Tel: 0531-88819269
WeChat Official Account
Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.
Business Email
E-mail: [email protected]
Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2
