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PMID: 6458044 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Genetic organization and transcriptional regulation in the qa gene cluster of Neurospora crassa.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 78 ·No. 9 ·1981-09-00 ·Pages 5783-7

Patel VB, Schweizer M, Dykstra CC, Kushner SR, Giles NH

Abstract

A transcription map of the qa gene cluster of Neurospora crassa has been constructed by using cloned DNA fragments as hybridization probes. The mRNAs encoded in the previously identified qa-2 (3-dehydroquinate hydro-lyase, EC 4.2.1.10), qa-4 (dehydroshikimate dehydratase), qa-3 (quinate:NAD+ 3-oxidoreductase, EC 1.1.1.24), and qa-1 (regulatory protein) genes have been characterized. In addition, mRNAs encoded in two new genes in this cluster (qa-x, qa-y) have been identified. Regulation of this system occurs at the level of transcription and is under the combined control of quinic acid and the qa-1 protein. The qa cluster represents a group of adjacent coding sequences which occupy approximately 18 kilobases in linkage group VII. The expression of the qa-1 gene appears to be constitutive but also autoregulated. mRNAs encoded in genes flanking the qa gene cluster have also been identified.

MeSH Terms
Gene Expression Regulation Genes Genes, Regulator Genetic Linkage Neurospora/genetics Neurospora crassa/genetics Quinic Acid/metabolism RNA, Messenger/genetics Transcription, Genetic
Chemicals
RNA, Messenger Quinic Acid
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Patel V B
Schweizer M
Dykstra C C
Kushner S R
Giles N H
References (16)
16 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.78.9.5783
Published
1981-09-00
Pages
5783-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC348861
Subset
IM
Grants
NIGMS NIH HHS · GM27997 · United States
NIGMS NIH HHS · GM28760 · United States
NIGMS NIH HHS · GM28777 · United States
External Links
PubMed source DOI: 10.1073/pnas.78.9.5783 PMC Full Text View journal details
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