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PMID: 6458274 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Sequence of a secondary phage lambda attachment site located between the pentitol operons of Klebsiella aerogenes.

The Biochemical journal ·Vol. 193 ·No. 2 ·1981-02-01 ·Pages 631-7

Loviny T, Neuberger MS, Hartley BS

Abstract

We have determined the nucleotide sequence of a secondary phage lambda attachment site (att) located between the structural genes of the ribitol and D-arabitol catabolic operons of Klebsiella aerogenes. The core region of this secondary attachment site (sequence: GGTTTTTTCGATTAT) shows considerable homology with the 15-base-pair core region common to both the phage att and the primary bacterial att of Escherichia coli K12 (sequence: GCTTTTTTACTAA); however, there is no such clear homology between the sequences flanking the cores of the primary att and this secondary att. Integration of phage lambda into the K. aerogenes secondary att occurred by recombination between the core region of the phage att and an oligo(T.A) stretch located within the K. aerogenes secondary att.

MeSH Terms
Attachment Sites, Microbiological Bacteriophage lambda/genetics,metabolism Base Sequence DNA, Bacterial/metabolism Klebsiella pneumoniae/analysis,genetics Lysogeny Operon Ribitol/metabolism Sugar Alcohols/metabolism Transduction, Genetic
Chemicals
DNA, Bacterial Sugar Alcohols Ribitol arabitol
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Loviny T
Neuberger M S
Hartley B S
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24 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1930631
Published
1981-02-01
Pages
631-7
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1162642
Subset
IM
Databases
GENBANK
M24339, M24340
External Links
PubMed source DOI: 10.1042/bj1930631 PMC Full Text View journal details
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