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PMID: 6481304 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Defective membrane potential changes in neutrophils from human neonates.

The Journal of experimental medicine ·Vol. 160 ·No. 4 ·1984-10-01 ·Pages 1247-52

Sacchi F, Hill HR

Abstract

In an attempt to determine the mechanism of the profound defect in chemotaxis observed in the polymorphonuclear leukocytes (PMN) of human neonates, we have examined membrane potential changes and alterations in free intracellular calcium following chemotactic factor stimulation. Following exposure to formyl-methionyl-leucyl-phenylalanine (FMLP), PMN from adult donors (11) showed a marked change in membrane potential (31%) as determined by fluorescence emission using the cyanine dye, 3-3-dipentyloxacarbocyanine [DiOC5(3)]. In marked contrast, FMLP-stimulated PMN from 10 human neonates failed to show any significant change in membrane potential (1-2%). Using the calcium-sensitive probe Quin 2/AM, FMLP induced an increase in fluorescence of up to 51% in adult PMN (10). In contrast, the change in intracellular free calcium induced in neonatal PMN was much less (32%; P less than 0.01). These results suggest that the profound defect in chemotactic responsiveness of PMN from human neonates may result from an inability of these cells to undergo changes in membrane potential following inflammatory mediator stimulation.

MeSH Terms
Adult Aminoquinolines Carbocyanines Cell Membrane/drug effects,metabolism Chemotaxis, Leukocyte Fetal Blood/cytology Fluorescent Dyes Humans Infant, Newborn Membrane Potentials/drug effects N-Formylmethionine Leucyl-Phenylalanine/pharmacology Neutrophils/metabolism Verapamil/pharmacology
Chemicals
Aminoquinolines Carbocyanines Fluorescent Dyes N-Formylmethionine Leucyl-Phenylalanine 3,3'-dipentyl-2,2'-oxacarbocyanine Quin2-acetoxymethyl ester Verapamil
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Sacchi F
Hill H R
References (18)
18 references, click to expand
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.160.4.1247
Published
1984-10-01
Pages
1247-52
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2187475
Subset
IM
Grants
NIAID NIH HHS · AI 13150 · United States
NIAID NIH HHS · AI 19094 · United States
External Links
PubMed source DOI: 10.1084/jem.160.4.1247 PMC Full Text View journal details
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