Abstract
The cleavage maps of a Staphylococcus aureus plasmid, pSN1 (2.75 megadaltons), conferring tetracycline resistance, were determined. Cleavage maps are given for HpaI and HindIII restriction endonucleases by using the single HpaII site as a reference point. Nucleases EcoRI, BamHI, SalI, and HaeIII have no sites on this plasmid.
MeSH Terms
DNA Restriction Enzymes/metabolism
DNA, Bacterial/analysis,metabolism
Drug Resistance, Microbial
Plasmids
Staphylococcus aureus/drug effects,genetics
Tetracycline/pharmacology
Chemicals
DNA, Bacterial
DNA Restriction Enzymes
Tetracycline
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Shafferman A
Shalita Z
Hertman I
References (6)
6 references, click to expand
-
Replication and expression of plasmids from Staphylococcus aureus in Bacillus subtilis.
Proc Natl Acad Sci U S A. 1977 Apr;74(4):1680-2
PMID: 404641
-
Isolation and characterization of a plasmid involved with enterotoxin B production in Staphylococcus aureus.
J Bacteriol. 1977 Jan;129(1):317-25
PMID: 830641
-
Construction of a colicin E1-R factor composite plasmid in vitro: means for amplification of deoxyribonucleic acid.
J Bacteriol. 1975 Jan;121(1):354-62
PMID: 1090574
-
Phage lambda receptor chromosomes for DNA fragments made with restriction endonuclease III of Haemophilus influenzae and restriction endonuclease I of Escherichia coli.
J Mol Biol. 1975 Nov 5;98(3):551-64
PMID: 1104875
-
Plasmid-protein relaxation complexes in Staphylococcus aureus.
J Bacteriol. 1976 Sep;127(3):1177-87
PMID: 956124
-
Isolation of a sequence-specific endonuclease (BamI) from Bacillus amyloliquefaciens H.
J Mol Biol. 1975 Sep 5;97(1):123-5
PMID: 1177312