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PMID: 6501422 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Acyl-Coa oxidase and hydratase-dehydrogenase, two enzymes of the peroxisomal beta-oxidation system, are synthesized on free polysomes of clofibrate-treated rat liver.

The Journal of cell biology ·Vol. 99 ·No. 6 ·1984-12-00 ·Pages 2241-6

Rachubinski RA, Fujiki Y, Mortensen RM, Lazarow PB

Abstract

We investigated the site of synthesis of two abundant proteins in clofibrate-induced rat hepatic peroxisomes. RNA was extracted from free and membrane-bound polysomes, heated to improve translational efficiency, and translated in the mRNA-dependent, reticulocyte-lysate-cell-free, protein-synthesizing system. The peroxisomal acyl-CoA oxidase and enoyl-CoA hydratase-beta-hydroxyacyl-CoA dehydrogenase 35S-translation products were isolated immunochemically, analyzed by SDS PAGE and fluorography, and quantitated by densitometric scanning. The RNAs coding for these two peroxisomal proteins were found predominantly on free polysomes, and the translation products co-migrated with the mature proteins. As in normal rat liver, preproalbumin and catalase were synthesized mainly by membrane-bound and by free polysomes, respectively. mRNAs for a number of minor 35S-translation products also retained by the anti-peroxisomal immunoadsorbent were similarly found on free polysomes. These results, together with previous data, allow the generalization that the content proteins of rat liver peroxisomes are synthesized on free polysomes, and the data imply a posttranslational packaging mechanism for these major content proteins.

MeSH Terms
Acyl-CoA Oxidase Animals Clofibrate/pharmacology Enoyl-CoA Hydratase/biosynthesis,genetics Hydro-Lyases/genetics Kinetics Liver/drug effects,enzymology Male Microbodies/drug effects,enzymology Molecular Weight Oxidoreductases/biosynthesis,genetics Polyribosomes/enzymology Protein Biosynthesis/drug effects RNA, Messenger/genetics Rats Rats, Inbred F344 Reticulocytes/metabolism
Chemicals
RNA, Messenger Oxidoreductases Acyl-CoA Oxidase Hydro-Lyases Enoyl-CoA Hydratase Clofibrate
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Rachubinski R A
Fujiki Y
Mortensen R M
Lazarow P B
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36 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.99.6.2241
Published
1984-12-00
Pages
2241-6
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2113575
Subset
IM
Grants
NIADDK NIH HHS · AM 19394 · United States
External Links
PubMed source DOI: 10.1083/jcb.99.6.2241 PMC Full Text View journal details
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