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PMID: 6538481 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Four secretory proteins synthesized by hepatocytes are transported from endoplasmic reticulum to Golgi complex at different rates.

The EMBO journal ·Vol. 3 ·No. 1 ·1984-01-00 ·Pages 147-52

Fries E, Gustafsson L, Peterson PA

Abstract

Pulse-chase experiments in conjunction with subcellular fractionation and quantitative immunoprecipitation have been used to study the intracellular transport of four secretory proteins, albumin, transferrin, prealbumin and retinol-binding protein, in isolated rat hepatocytes. The proteins were found to be transported from the endoplasmic reticulum (ER) to the Golgi complex (GC) at greatly different rates (t1/2 = 14-137 min), indicating that transport of secretory proteins between these organelles is effected by a selective, possibly receptor-mediated process and not through bulk phase transfers. The transport from the Golgi complex to the medium was rapid for all proteins (t1/2 approximately 15 min) and possibly occurred at the same rate. Consistent with these kinetic data, the amount of a rapidly transported protein (albumin) in the GC fraction was found to be high (relative to its amount in the ER fraction) whereas the amount of a slowly transported protein (transferrin) in the GC fraction was found to be low, as determined by radioimmunoassays.

MeSH Terms
Animals Cell Fractionation Endoplasmic Reticulum/metabolism Golgi Apparatus/metabolism Kinetics Liver/metabolism Male Prealbumin/genetics Protein Processing, Post-Translational Proteins/genetics Rats Rats, Inbred Strains Retinol-Binding Proteins/genetics Serum Albumin/genetics Subcellular Fractions/metabolism Transferrin/genetics
Chemicals
Prealbumin Proteins Retinol-Binding Proteins Serum Albumin Transferrin
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Fries E
Gustafsson L
Peterson P A
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35 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1984-01-00
Pages
147-52
Language
English
Region
England
NLM ID
8208664
PMCID
PMC557311
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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