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PMID: 6561518 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Six Schizosaccharomyces pombe tRNA genes including a gene for a tRNALys with an intervening sequence which cannot base-pair with the anticodon.

Nucleic acids research ·Vol. 11 ·No. 24 ·1983-12-20 ·Pages 8537-46

Gamulin V, Mao J, Appel B, Sumner-Smith M, Yamao F, Söll D

Abstract

We report the sequences of six S. pombe tRNA genes including two genes for tRNAArg, and one gene each for tRNAGlu, tRNAHis, tRNALys and tRNAPhe. All tRNA genes are found independently in the genome and represent individual transcription units. The gene for tRNALys has an 8 bp long intervening sequence which cannot base-pair with the tRNA anticodon. In vitro transcription studies indicate that all genes are faithfully transcribed in a yeast extract. Sequence comparison of the 5' flanking regions of the tRNA genes did not show significant homologies; however, they are very rich in AT base pairs.

MeSH Terms
Anticodon Ascomycota/genetics Base Composition Base Sequence Cloning, Molecular Genes, Fungal Nucleic Acid Conformation RNA, Transfer/genetics RNA, Transfer, Amino Acyl/genetics Schizosaccharomyces/genetics Transcription, Genetic
Chemicals
Anticodon RNA, Transfer, Amino Acyl RNA, Transfer
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Gamulin V
Mao J
Appel B
Sumner-Smith M
Yamao F
Söll D
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/11.24.8537
Published
1983-12-20
Pages
8537-46
Language
English
Region
England
NLM ID
0411011
PMCID
PMC326605
Subset
IM
Databases
GENBANK
X00239, X00240, X00241, X00242, X00283
External Links
PubMed source DOI: 10.1093/nar/11.24.8537 PMC Full Text View journal details
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