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PMID: 6582504 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

On the mechanism of nucleolar dominance in mouse-human somatic cell hybrids.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 81 ·No. 2 ·1984-01-00 ·Pages 484-7

Onishi T, Berglund C, Reeder RH

Abstract

The mechanism of nucleolar dominance was studied in two lines of mouse-human somatic hybrids. Both lines had preferentially lost human chromosomes but had retained significant amounts of both mouse and human ribosomal genes (genes coding for the 18S, 5.8S, and 28S RNAs of ribosomes). However, the human ribosomal genes were repressed, and only mouse ribosomal genes were expressed. Soluble transcription extracts from the hybrids were able to initiate RNA synthesis accurately on a cloned mouse ribosomal gene but were unable to initiate accurately on a human ribosomal gene. This suggests that nucleolar dominance in these hybrids is due to the loss or inactivation of the gene for a specificity factor required to recognize the human ribosomal gene promoter.

MeSH Terms
Animals Gene Expression Regulation Genes Genes, Dominant Humans Hybrid Cells/physiology Mice Operon RNA, Ribosomal/genetics Transcription Factors/physiology Transcription, Genetic
Chemicals
RNA, Ribosomal Transcription Factors
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Onishi T
Berglund C
Reeder R H
References (25)
25 references, click to expand
  1. Preferential transcription of Xenopus laevis ribosomal RNA in interspecies hybrids between Xenopus laevis and Xenopus mulleri.
    J Mol Biol. 1973 Oct 25;80(2):217-28 PMID: 4763983
  2. Spacer regulation of Xenopus ribosomal gene transcription: competition in oocytes.
    Cell. 1983 Dec;35(2 Pt 1):449-56 PMID: 6652676
  3. Expression of human and suppression of mouse nucleolus organizer activity in mouse-human somatic cell hybrids.
    Proc Natl Acad Sci U S A. 1976 Dec;73(12):4531-5 PMID: 1070003
  4. Suppression of production of mouse 28S ribosomal RNA in mouse-human hybrids segregating mouse chromosomes.
    Proc Natl Acad Sci U S A. 1977 Feb;74(2):694-7 PMID: 265531
  5. Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange.
    Proc Natl Acad Sci U S A. 1977 Nov;74(11):4835-8 PMID: 73185
  6. Selective suppression of the transcription of ribosomal genes in mouse-human hybrid cells.
    J Cell Physiol. 1979 Mar;98(3):553-9 PMID: 438299
  7. Reactivation of silent rRNA genes by simian virus 40 in human-mouse hybrid cells.
    Proc Natl Acad Sci U S A. 1979 Aug;76(8):3885-9 PMID: 226986
  8. Selective and accurate initiation of transcription at the Ad2 major late promotor in a soluble system dependent on purified RNA polymerase II and DNA.
    Cell. 1979 Oct;18(2):469-84 PMID: 498279
  9. Reactivation of ribosomal RNA genes in human-mouse hybrid cells by 12-O-tetradecanoylphorbol 13-acetate.
    Proc Natl Acad Sci U S A. 1980 Mar;77(3):1566-9 PMID: 6246494
  10. DNA-dependent transcription of adenovirus genes in a soluble whole-cell extract.
    Proc Natl Acad Sci U S A. 1980 Jul;77(7):3855-9 PMID: 6933441
  11. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.
    Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201-5 PMID: 6159641
  12. Specific transcription of mouse ribosomal DNA in a cell-free system that mimics control in vivo.
    Proc Natl Acad Sci U S A. 1981 Feb;78(2):727-31 PMID: 6262766
  13. Transcription of mouse rRNA genes by RNA polymerase I: in vitro and in vivo initiation and processing sites.
    Cell. 1981 Nov;27(1 Pt 2):165-74 PMID: 7326749
  14. Ribosomal RNA transcription in vitro is species specific.
    Nature. 1982 Mar 11;296(5853):173-4 PMID: 7063022
  15. Fate of amplified nucleoli in Xenopus laevis embryos.
    Dev Biol. 1982 Jun;91(2):458-67 PMID: 7201429
  16. Human ribosomal RNA gene: nucleotide sequence of the transcription initiation region and comparison of three mammalian genes.
    Proc Natl Acad Sci U S A. 1982 May;79(10):3092-6 PMID: 6954460
  17. DNAase I sensitivity and methylation of active versus inactive rRNA genes in xenopus species hybrids.
    Cell. 1982 May;29(1):211-8 PMID: 6286140
  18. Identification of the in vivo and in vitro origin of transcription in human rDNA.
    Nucleic Acids Res. 1982 Jul 10;10(13):3933-49 PMID: 6287426
  19. Transcription of Xenopus borealis rRNA genes in nuclei of Xenopus laevis oocytes.
    Dev Biol. 1982 Oct;93(2):471-7 PMID: 7141111
  20. Transcription of Xenopus ribosomal RNA genes by RNA polymerase I in vitro.
    J Biol Chem. 1982 Dec 10;257(23):14375-83 PMID: 7142215
  21. Fractionation and reconstitution of factors required for accurate transcription of mammalian ribosomal RNA genes: identification of a species-dependent initiation factor.
    Nucleic Acids Res. 1982 Nov 11;10(21):6659-70 PMID: 7177852
  22. In vitro transcription of human ribosomal RNA genes by RNA polymerase I.
    J Mol Appl Genet. 1982;1(6):575-84 PMID: 7153690
  23. Mutational analysis of simian virus 40 T antigen: stimulation of cellular DNA synthesis and activation of rRNA genes by mutants with deletions in the T-antigen gene.
    Mol Cell Biol. 1983 Feb;3(2):214-9 PMID: 6300657
  24. Spacer sequences regulate transcription of ribosomal gene plasmids injected into Xenopus embryos.
    Cell. 1983 Oct;34(3):989-96 PMID: 6627394
  25. Nucleotide sequence of the rightward operator of phage lambda.
    Proc Natl Acad Sci U S A. 1975 Mar;72(3):1184-8 PMID: 1055375
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.81.2.484
Published
1984-01-00
Pages
484-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC344702
Subset
IM
Grants
NIADDK NIH HHS · AM33345 · United States
NIGMS NIH HHS · GM26624 · United States
External Links
PubMed source DOI: 10.1073/pnas.81.2.484 PMC Full Text View journal details
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