Home LiteratureArticle Details
PMID: 6586713 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Mapping of Rhodopseudomonas capsulata nif genes.

Journal of bacteriology ·Vol. 158 ·No. 2 ·1984-05-00 ·Pages 404-10

Wall JD, Braddock K

Abstract

The endogenous gene transfer system of Rhodopseudomonas capsulata was used to analyze mutations which block the ability to use molecular nitrogen as the sole nitrogen source (nif). With this fine-structure mapping tool, linkage of nif mutations could be reliably established if separated by 2,700 base pairs or less. Eleven independent mutations were analyzed, and five linkage groups were found. The overall chromosomal arrangement of these groups awaits conjugational or physical analysis. A candidate for the inactive subunit of R. capsulata Fe protein was located in gels at a position of about 38,000 molecular weight, 5,000 more than that of the presumed active subunit.

MeSH Terms
Chromosome Mapping Chromosomes, Bacterial Genes, Bacterial Genetic Complementation Test Genetic Linkage Mutation Nitrogen Fixation Nitrogenase/analysis,genetics,metabolism Rhodopseudomonas/enzymology,genetics,metabolism
Chemicals
Nitrogenase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Wall J D
Braddock K
References (24)
24 references, click to expand
  1. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  2. Nitrogenase from the photosynthetic bacterium Rhodopseudomonas capsulata: purification and molecular properties.
    J Bacteriol. 1982 Feb;149(2):708-17 PMID: 6799495
  3. Genetics and regulation of nitrogen fixation.
    Annu Rev Microbiol. 1981;35:207-35 PMID: 7027900
  4. Interactions of heterologous nitrogenase components that generate catalytically inactive complexes.
    Proc Natl Acad Sci U S A. 1976 Dec;73(12):4369-73 PMID: 1069989
  5. Characterization of the gene transfer agent made by an overproducer mutant of Rhodopseudomonas capsulata.
    J Mol Biol. 1979 Jun 25;131(2):157-68 PMID: 490646
  6. Alignment of genetic and restriction maps of the photosynthesis region of the Rhodopseudomonas capsulata chromosome by a conjugation-mediated marker rescue technique.
    J Bacteriol. 1983 May;154(2):580-90 PMID: 6302077
  7. Inhibition of nitrogenase activity by NH+4 in Rhodospirillum rubrum.
    J Bacteriol. 1981 Feb;145(2):824-31 PMID: 6780531
  8. Interspecies homology of nitrogenase genes.
    Proc Natl Acad Sci U S A. 1980 Jan;77(1):191-5 PMID: 6987649
  9. Phosphorylation-dephosphorylation of pyruvate dehydrogenase complex from pea leaf mitochondria.
    Arch Biochem Biophys. 1981 Apr 1;207(2):437-44 PMID: 7247414
  10. A pleiotropic mutant of Rhodopseudomonas capsulata defective in nitrogen metabolism.
    Arch Microbiol. 1977 Dec 15;115(3):259-63 PMID: 23732
  11. Regulation of nitrogenase in the photosynthetic bacterium Rhodopseudomonas capsulata as studied by two-dimensional gel electrophoresis.
    J Bacteriol. 1982 Sep;151(3):1612-6 PMID: 6955301
  12. Genetic and physical map of the structural genes (nifH,D,K) coding for the nitrogenase complex of Rhodopseudomonas capsulata.
    J Bacteriol. 1983 Oct;156(1):251-6 PMID: 6311797
  13. Regulation of nitrogenase synthesis in intact cells of Rhodospirillum rubrum: inactivation of nitrogen fixation by ammonia, L-glutamine and L-asparagine.
    J Gen Microbiol. 1975 Nov;91(1):53-62 PMID: 811763
  14. Comparison of active and inactive forms of iron protein from Rhodospirillum rubrum.
    Biochem J. 1982 Jun 1;203(3):663-8 PMID: 6810874
  15. Purification and properties of nitrogenase from Rhodospirillum rubrum, and evidence for phosphate, ribose and an adenine-like unit covalently bound to the iron protein.
    Biochem J. 1978 Oct 1;175(1):251-9 PMID: 104713
  16. Biochemistry and genetics of Klebsiella pneumoniae mutant strains unable to fix N2.
    J Bacteriol. 1975 Mar;121(3):759-65 PMID: 1090602
  17. Genetic recombination in Rhodopseudomonas capsulata.
    Proc Natl Acad Sci U S A. 1974 Mar;71(3):971-3 PMID: 4522805
  18. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  19. Derepression of nitrogenase activity in glutamine auxotrophs of Rhodopseudomonas capsulata.
    J Bacteriol. 1979 Mar;137(3):1459-63 PMID: 35518
  20. Regulation and characterization of protein products coded by the nif (nitrogen fixation) genes of Klebsiella pneumoniae.
    J Bacteriol. 1978 Oct;136(1):267-79 PMID: 361694
  21. Genetic transfer of nitrogenase-hydrogenase activity in Rhodopseudomonas capsulata.
    Nature. 1975 Dec 18;258(5536):630-1 PMID: 1207743
  22. Map of genes for carotenoid and bacteriochlorophyll biosynthesis in Rhodopseudomonas capsulata.
    J Bacteriol. 1976 May;126(2):619-29 PMID: 1262313
  23. Identification of blue-green algal nitrogen fixation genes by using heterologous DNA hybridization probes.
    Proc Natl Acad Sci U S A. 1980 Jan;77(1):186-90 PMID: 16592748
  24. Removal of an adenine-like molecule during activation of dinitrogenase reductase from Rhodospirillum rubrum.
    Proc Natl Acad Sci U S A. 1979 Dec;76(12):6201-5 PMID: 118462
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.158.2.404-410.1984
Published
1984-05-00
Pages
404-10
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC215442
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.158.2.404-410.1984 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2