Vasopressin gene is expressed at low levels in the hypothalamus of the Brattleboro rat.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 81 ·No. 17 ·1984-09-00 ·Pages 5296-9

Majzoub JA, Pappey A, Burg R, Habener JF

Abstract

The Brattleboro rat carries as a recessive trait the inability to synthesize hypothalamic vasopressin and its related neurophysin but is able to synthesize oxytocin and its neurophysin. Brattleboro rats homozygous for this trait have no immunologically detectable circulating vasopressin and manifest a complete syndrome of diabetes insipidus, which is corrected with vasopressin replacement therapy. Such a defect could be due to absence of the gene encoding vasopressin, the presence of an abnormal gene, or a variety of transcriptional or posttranscriptional abnormalities. We report here that the hypothalamus of the Brattleboro rat contains detectable, although markedly reduced, levels of an mRNA indistinguishable in size with and similar in sequence to authentic vasopressin mRNA. Corresponding levels of oxytocin mRNA were the same in Brattleboro and normal rat hypothalami. These findings indicate that the Brattleboro rat expresses a vasopressin gene, but at a reduced level.

MeSH Terms

Amino Acid Sequence Animals Base Sequence DNA/analysis Female Genes Hypothalamus/metabolism Nucleic Acid Hybridization RNA, Messenger/genetics Rats Rats, Brattleboro/genetics Rats, Mutant Strains/genetics Vasopressins/genetics

Chemicals

RNA, Messenger Vasopressins DNA

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Majzoub J A

Pappey A

Burg R

Habener J F

References (23)

23 references, click to expand

Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose.
Proc Natl Acad Sci U S A. 1972 Jun;69(6):1408-12 PMID: 4504350
Vasopressin, tissue-specific defects and the Brattleboro rat.
Nature. 1984 Jul 5-11;310(5972):17 PMID: 6738699
Detection and partial sequence analysis of gastrin mRNA by using an oligodeoxynucleotide probe.
Proc Natl Acad Sci U S A. 1979 Apr;76(4):1770-4 PMID: 88048
Transcriptional regulation of the ovalbumin and conalbumin genes by steroid hormones in chick oviduct.
J Biol Chem. 1979 Sep 25;254(18):9050-8 PMID: 479179
Hybridization of synthetic oligodeoxyribonucleotides to phi chi 174 DNA: the effect of single base pair mismatch.
Nucleic Acids Res. 1979 Aug 10;6(11):3543-57 PMID: 158748
Synthesis, transport, and release of posterior pituitary hormones.
Science. 1980 Jan 25;207(4429):373-8 PMID: 6153132
Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.
Biochemistry. 1979 Nov 27;18(24):5294-9 PMID: 518835
Gel electrophoresis of restriction fragments.
Methods Enzymol. 1979;68:152-76 PMID: 232210
Sequencing end-labeled DNA with base-specific chemical cleavages.
Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.
Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201-5 PMID: 6159641
Nucleotide sequence of cloned cDNA encoding bovine arginine vasopressin-neurophysin II precursor.
Nature. 1982 Jan 28;295(5847):299-303 PMID: 6276766
Abnormal RNA processing due to the exon mutation of beta E-globin gene.
Nature. 1982 Dec 23;300(5894):768-9 PMID: 7177196
The hormonal status of the Brattleboro rat.
Ann N Y Acad Sci. 1982;394:535-48 PMID: 6295231
Precursors in the biosynthesis of vasopressin and oxytocin in the rat. Characteristics of all the components in high-performance liquid chromatography.
Biochem J. 1982 Nov 15;208(2):339-49 PMID: 7159402
Gene conversion and polymorphism: generation of mouse immunoglobulin gamma 2a chain alleles by differential gene conversion by gamma 2b chain gene.
Cell. 1983 Feb;32(2):515-23 PMID: 6297797
Deduced amino acid sequence from the bovine oxytocin-neurophysin I precursor cDNA.
Nature. 1983 Mar 24-30;302(5906):342-4 PMID: 6687626
Specific transcription and RNA splicing defects in five cloned beta-thalassaemia genes.
Nature. 1983 Apr 14;302(5909):591-6 PMID: 6188062
Nucleotide polymorphism at the alcohol dehydrogenase locus of Drosophila melanogaster.
Nature. 1983 Aug 4-10;304(5925):412-7 PMID: 6410283
Polymorphism near the rat prolactin gene caused by insertion of an Alu-like element.
Nature. 1983 Sep 8-14;305(5930):159-60 PMID: 6310412
Structural organization of the rat gene for the arginine vasopressin-neurophysin precursor.
EMBO J. 1983;2(5):763-7 PMID: 6315416
Vasopressin and oxytocin mRNA regulation in the rat assessed by hybridization with synthetic oligonucleotides.
J Biol Chem. 1983 Dec 10;258(23):14061-4 PMID: 6689019
Single base deletion in the vasopressin gene is the cause of diabetes insipidus in Brattleboro rats.
Nature. 1984 Apr 19-25;308(5961):705-9 PMID: 6717565
A new method for sequencing DNA.
Proc Natl Acad Sci U S A. 1977 Feb;74(2):560-4 PMID: 265521

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.81.17.5296

Published

1984-09-00

Pages

5296-9

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC391690

Subset

Databases

GENBANK

K02434

External Links

PubMed source DOI: 10.1073/pnas.81.17.5296 PMC Full Text View journal details

Analysis Services

Vasopressin gene is expressed at low levels in the hypothalamus of the Brattleboro rat.

Contact

WeChat Official Account