Home LiteratureArticle Details
PMID: 6609682 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Purification and properties of an inducible cephalosporinase from Pseudomonas maltophilia GN12873.

Antimicrobial agents and chemotherapy ·Vol. 25 ·No. 3 ·1984-03-00 ·Pages 362-5

Saino Y, Inoue M, Mitsuhashi S

Abstract

An inducible cephalosporinase was purified from Pseudomonas maltophilia GN12873. The pI was 8.4, and the molecular weight was ca. 56,000 by gel filtration or 27,000 by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, suggesting that this enzyme had two subunits. The optimal pH and optimal temperature were 7.5 and 45 degrees C, respectively. Enzyme activity was inhibited by clavulanic acid, sulbactam, cephamycin derivatives, carbapenem antibiotics, iodine, HgCl2, and p-chloromercuribenzoate. The enzyme showed a broad substrate profile, hydrolyzing cephaloridine, cefazolin, cefsulodin, penicillin G, ceftizoxime, and ampicillin at a high rate.

MeSH Terms
Anti-Bacterial Agents/pharmacology Bacterial Proteins/isolation & purification Cephalosporinase/isolation & purification,metabolism Chemical Phenomena Chemistry, Physical Chromatography, Gel Enzyme Induction/drug effects Hydrogen-Ion Concentration Pseudomonas/enzymology beta-Lactamase Inhibitors beta-Lactamases/metabolism
Chemicals
Anti-Bacterial Agents Bacterial Proteins beta-Lactamase Inhibitors Cephalosporinase beta-Lactamases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Saino Y
Inoue M
Mitsuhashi S
References (18)
18 references, click to expand
  1. Estimation of the molecular weights of proteins by Sephadex gel-filtration.
    Biochem J. 1964 May;91(2):222-33 PMID: 4158310
  2. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  3. Maturation of the head of bacteriophage T4. I. DNA packaging events.
    J Mol Biol. 1973 Nov 15;80(4):575-99 PMID: 4204102
  4. Beta-lactamases from Yersinia enterocolitica.
    J Gen Microbiol. 1975 Apr;87(2):273-84 PMID: 237976
  5. The dimeric nature of an R-factor mediated beta-lactamase.
    Biochem Biophys Res Commun. 1976 Feb 9;68(3):1000-5 PMID: 769788
  6. The beta-lactamases of gram-negative bacteria and their role in resistance to beta-lactam antibiotics.
    J Antimicrob Chemother. 1976 Jun;2(2):115-57 PMID: 783110
  7. Susceptibility of Pseudomonas maltophilia to antimicrobial agents, singly and in combination.
    Antimicrob Agents Chemother. 1979 Dec;16(6):833-7 PMID: 533264
  8. Activity of beta-lactamase produced by Bacteroides fragilis against newly introduced cephalosporins.
    Antimicrob Agents Chemother. 1980 Apr;17(4):736-7 PMID: 6967295
  9. Antibacterial activities of a new stabilized thienamycin, N-formimidoyl thienamycin, in comparison with other antibiotics.
    Antimicrob Agents Chemother. 1980 Jun;17(6):912-7 PMID: 6931548
  10. Purification and properties of a new beta-lactamase from Pseudomonas cepacia.
    Antimicrob Agents Chemother. 1980 Mar;17(3):355-8 PMID: 6968539
  11. Purification and some properties of a cephalosporinase from Proteus vulgaris.
    Antimicrob Agents Chemother. 1981 Jan;19(1):185-7 PMID: 7018383
  12. Beta-lactamase stability and antibacterial activity of cefmenoxime (SCE-1365), a novel cephalosporin.
    Antimicrob Agents Chemother. 1981 Aug;20(2):171-5 PMID: 6269488
  13. Comparative in vitro activity of N-formimidoyl thienamycin against gram-positive and gram-negative aerobic and anaerobic species and its beta-lactamase stability.
    Antimicrob Agents Chemother. 1982 Jan;21(1):180-7 PMID: 6979306
  14. Azthreonam (SQ 26,776), a synthetic monobactam specifically active against aerobic gram-negative bacteria.
    Antimicrob Agents Chemother. 1982 Jan;21(1):85-92 PMID: 6979307
  15. Interaction of azthreonam and related monobactams with beta-lactamases from gram-negative bacteria.
    Antimicrob Agents Chemother. 1982 Sep;22(3):414-20 PMID: 6982680
  16. Purification and properties of inducible penicillin beta-lactamase isolated from Pseudomonas maltophilia.
    Antimicrob Agents Chemother. 1982 Oct;22(4):564-70 PMID: 6983856
  17. In vitro activity of azthreonam, a monobactam antibiotic.
    Antimicrob Agents Chemother. 1982 Nov;22(5):832-8 PMID: 6891198
  18. The beta-lactamases of gram-negative bacteria and their possible physiological role.
    Adv Microb Physiol. 1973;9:31-88 PMID: 4581138
Article Info
Journal
Antimicrobial agents and chemotherapy
Abbr.
Antimicrob Agents Chemother
ISSN
0066-4804
DOI
10.1128/AAC.25.3.362
Published
1984-03-00
Pages
362-5
Language
English
Region
United States
NLM ID
0315061
PMCID
PMC185518
Subset
IM
External Links
PubMed source DOI: 10.1128/AAC.25.3.362 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2