Primer recognition by avian myeloblastosis virus RNA-directed DNA polymerase.

Journal of virology ·Vol. 21 ·No. 3 ·1977-03-00 ·Pages 1236-9

Brown RD, Armentrout RW

Abstract

Tryptophanyl-tRNA was specifically labeled at the 3' end with [3H]tryptophan and cleaved in half with RNase under denaturing conditions, and the 3' half was shown to hybridize exclusively at the 5' end of avian myeloblastosis virus RNA. The RNA-dependent DNA polymerase of avian myeloblastosis virus is capable of efficiently binding the 3' half of the primer molecule.

MeSH Terms

Avian Leukosis Virus/enzymology Avian Myeloblastosis Virus/enzymology Binding Sites Nucleic Acid Hybridization RNA, Transfer/metabolism RNA, Viral/metabolism RNA-Directed DNA Polymerase/metabolism

Chemicals

RNA, Viral RNA, Transfer RNA-Directed DNA Polymerase

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Brown R D

Armentrout R W

References (12)

12 references, click to expand

Size of the polyadenylic acid region of newly synthesized globin messenger ribonucleic acid.
J Biol Chem. 1975 May 25;250(10):3725-8 PMID: 1126933
A primer ribonucleic acid for initiation of in vitro Rous sarcarcoma virus deoxyribonucleic acid synthesis.
J Biol Chem. 1975 May 10;250(9):3487-97 PMID: 164470
Site on the RNA of an avian sarcoma virus at which primer is bound.
J Virol. 1975 Sep;16(3):553-8 PMID: 169390
Interaction of tryptophan transfer RNA with Rous sarcoma virus 35S RNA.
Nature. 1976 Jan 22;259(5540):245-7 PMID: 175281
Initiation sites of Rous sarcoma virus RNA-directed DNA synthesis in vitro.
Nature. 1976 JUL 15;262(5565):186-90 PMID: 180433
Nucleotide sequence that binds primer for DNA synthesis to the avian sarcoma virus genome.
J Virol. 1976 Aug;19(2):548-58 PMID: 183013
Transcription of DNA from the 70S RNA of Rous sarcoma virus. I. Identification of a specific 4S RNA which serves as primer.
J Virol. 1974 May;13(5):1126-33 PMID: 4132919
Transcription of DNA from the 70S RNA of Rous sarcoma virus. II. Structure of a 4S RNA primer.
J Virol. 1974 May;13(5):1134-42 PMID: 4132920
RNA-directed DNA polymerase of Rous sarcoma virus: initiation of synthesis with 70 S viral RNA as template.
J Mol Biol. 1973 Sep 5;79(1):163-83 PMID: 4355596
Molecular weight determination of nucleic acids by gel electrophoresis in non-aqueous solution.
Nat New Biol. 1972 Jan 26;235(56):108-10 PMID: 4551278
Transfer RNA coded by the T4 bacteriophage genome.
Proc Natl Acad Sci U S A. 1968 Sep;61(1):114-21 PMID: 4880604
Evidence for circularization of the avian oncornavirus RNA genome during proviral DNA synthesis from studies of reverse transcription in vitro.
Proc Natl Acad Sci U S A. 1976 Apr;73(4):1329-32 PMID: 57620

Article Info

Journal

Journal of virology

Abbr.

J Virol

ISSN

0022-538X

DOI

10.1128/JVI.21.3.1236-1239.1977

Published

1977-03-00

Pages

1236-9

Language

English

Region

United States

NLM ID

0113724

PMCID

PMC515668

Subset

External Links

PubMed source DOI: 10.1128/JVI.21.3.1236-1239.1977 PMC Full Text View journal details

Analysis Services

Primer recognition by avian myeloblastosis virus RNA-directed DNA polymerase.

Contact

WeChat Official Account