Shuffling adenovirus promoters: a viral recombinant with early region 1A under late transcriptional control.

The EMBO journal ·Vol. 2 ·No. 6 ·1983-00-00 ·Pages 845-51

Solnick D

Abstract

Adenovirus recombinants were constructed in which a copy of the major late promoter was inserted either upstream or in place of the promoter for early region 1A (E1A). The ectopic late promoter directs a 20- to 100-fold increase in the level of cytoplasmic RNA from E1A. The RNA is correctly processed at the normal splice and polyadenylation sites, and directs the in vitro synthesis of the full complement of E1A proteins. Furthermore, the novel transcripts are translated in vivo, since the recombinants are fully viable on HeLa cells notwithstanding the loss in one case of the intact E1A promoter.

MeSH Terms

Adenoviridae/genetics Genes, Viral HeLa Cells Humans Operon Protein Biosynthesis RNA, Viral/genetics Recombination, Genetic Transcription, Genetic Viral Proteins/biosynthesis

Chemicals

RNA, Viral Viral Proteins

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Solnick D

References (44)

44 references, click to expand

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Article Info

Journal

The EMBO journal

Abbr.

EMBO J

ISSN

0261-4189

Published

1983-00-00

Pages

845-51

Language

English

Region

England

NLM ID

8208664

PMCID

PMC555199

Subset

External Links

PubMed source PMC Full Text View journal details

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Shuffling adenovirus promoters: a viral recombinant with early region 1A under late transcriptional control.

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