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PMID: 6672768 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Stabilization of (dG-dC)n.(dG-dC)n in the Z conformation by a crosslinking reaction.

Nucleic acids research ·Vol. 11 ·No. 23 ·1983-12-10 ·Pages 8421-9

Castleman H, Hanau LH, Erlanger BF

Abstract

(dG-dC)n.(dG-dC) was converted to the Z conformer by heating in the presence of Mn++n. Reaction of this preparation with the crosslinking reagent, DL-diepoxybutane (DEB), stabilized this conformer so that it retained its structure even when returned to conditions that favored reversion to the B conformation. Treatment of the crosslinked Z conformer with periodate caused scission of the crosslink, allowing reversion to the B conformer. Reaction of (dG-dC)n.(dG-dC)n in the B conformation with DEB did not prevent conversion to the Z conformer in 4M NaC1; dialysis of the high salt solution against low ionic strength buffer allowed return to the B conformer. The Z in equilibrium B transitions were followed by circular dichroism studies and immunochemical procedures. The results suggest the feasibility of stabilizing Z sequences of DNA in chromatin by crosslinking, so that they could then be identified after DNA isolation.

MeSH Terms
Circular Dichroism Drug Stability Epoxy Compounds Ethers, Cyclic Kinetics Manganese Mutagens Nucleic Acid Conformation Polydeoxyribonucleotides
Chemicals
Epoxy Compounds Ethers, Cyclic Mutagens Polydeoxyribonucleotides poly(dC-dG) Manganese diepoxybutane
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Castleman H
Hanau L H
Erlanger B F
References (24)
24 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/11.23.8421
Published
1983-12-10
Pages
8421-9
Language
English
Region
England
NLM ID
0411011
PMCID
PMC326592
Subset
IM
Grants
NIGMS NIH HHS · 5-T-32-GM-07367 · United States
NIAID NIH HHS · AI-06860 · United States
External Links
PubMed source DOI: 10.1093/nar/11.23.8421 PMC Full Text View journal details
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