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PMID: 6684661 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Cytoplasmic organization in cerebellar dendritic spines.

The Journal of cell biology ·Vol. 97 ·No. 4 ·1983-10-00 ·Pages 1169-78

Landis DM, Reese TS

Abstract

Three sets of filamentous structures were found to be associated with synaptic junctions in slices of cerebellar tissue prepared by rapid-freezing and freeze-etch techniques. The electron-dense fuzz subjacent to postsynaptic membranes corresponds to a web of 4-6-nm-diam filaments that were clearly visualized in rapid-frozen, freeze-etched preparations. Purkinje cell dendritic spines are filled with a meshwork of 5-7-nm filaments that were found to contact the spine membrane everywhere except at the synaptic junction, and extend through the neck of the spine into the parent dendrite. In addition, 8-10-nm microfilaments, possibly actin, were seen to be associated with the postsynaptic web and to extend into the body and neck of the spine. The arrangements and attachments of the filamentous elements in the Purkinje cell dendritic spine may account for its shape.

MeSH Terms
Animals Cytoplasm/ultrastructure Cytoplasmic Granules/ultrastructure Cytoskeleton/ultrastructure Dendrites/ultrastructure Freeze Fracturing Mice Mice, Inbred C57BL Microscopy, Electron Purkinje Cells/ultrastructure Synapses/ultrastructure Synaptic Vesicles/ultrastructure
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Landis D M
Reese T S
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46 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.97.4.1169
Published
1983-10-00
Pages
1169-78
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2112605
Subset
IM
Grants
NINDS NIH HHS · NS 00353 · United States
NINDS NIH HHS · NS 15573 · United States
External Links
PubMed source DOI: 10.1083/jcb.97.4.1169 PMC Full Text View journal details
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