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PMID: 6687941 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The vicilin gene family of pea (Pisum sativum L.): a complete cDNA coding sequence for preprovicilin.

Nucleic acids research ·Vol. 11 ·No. 8 ·1983-04-25 ·Pages 2367-80

Lycett GW, Delauney AJ, Gatehouse JA, Gilroy J, Croy RR, Boulter D

Abstract

A cDNA plasmid bank has been constructed using mRNA from developing pea seeds and three cDNAs coding for vicilin polypeptides have been selected. These cDNAs have been sequenced and between them cover the whole of the coding sequence plus part of the 5' and 3' untranslated regions. Comparison with amino acid sequence data from the protein indicates that vicilin is synthesised as preprovicilin with subsequent removal of a signal peptide and a C-terminal peptide as well as post translational endo-proteolytic cleavage. The cDNAs represent two different classes of vicilin genes whilst amino acid data show that there are at least three major classes of vicilin polypeptide. The vicilin sequences show extensive homology with conglycinin and phaseolin except in the regions of the internal proteolytic cleavages. The evolutionary significance of this relationship is discussed.

MeSH Terms
Amino Acid Sequence Base Sequence DNA/metabolism Genes Nucleic Acid Hybridization Plant Proteins Plant Proteins, Dietary/genetics Plants/genetics Plasmids Protein Biosynthesis Protein Processing, Post-Translational RNA, Messenger/genetics Seed Storage Proteins
Chemicals
Plant Proteins Plant Proteins, Dietary RNA, Messenger Seed Storage Proteins DNA vicilin protein, plant
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Lycett G W
Delauney A J
Gatehouse J A
Gilroy J
Croy R R
Boulter D
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/11.8.2367
Published
1983-04-25
Pages
2367-80
Language
English
Region
England
NLM ID
0411011
PMCID
PMC325890
Subset
IM
Databases
GENBANK
J01258, J01259, J01260
External Links
PubMed source DOI: 10.1093/nar/11.8.2367 PMC Full Text View journal details
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