Home LiteratureArticle Details
PMID: 6156379 Published · ppublish English Journal Article

Chloramphenicol releases a block in initiation of chromosome replication in a dnaA strain of Escherichia coli K12.

Molecular & general genetics : MGG ·Vol. 178 ·No. 2 ·1980-00-00 ·Pages 329-36

Lycett GW, Orr E, Pritchard RH

Abstract

DNA-DNA hybridisation experiments show that chloramphenicol induces a burst of initiation from the oriC region of a dnaA46 mutant of Escherichia coli at 36.5 degrees C but not from the isogenic dnaA+ strain. Following this stimulation of initiation is in parallel with the induced stimulation of RNA synthesis caused by chloramphenicol in the same strain. This is consistent with the hypothesis that the stimulation of initiation in the dnaA mutant is the result of the stimulation of the synthesis of an RNA species.

MeSH Terms
Chloramphenicol/pharmacology Chromosomes, Bacterial DNA Replication/drug effects DNA, Bacterial/genetics Escherichia coli/genetics Mutation Nucleic Acid Hybridization Plasmids RNA, Bacterial/biosynthesis Stimulation, Chemical
Chemicals
DNA, Bacterial RNA, Bacterial Chloramphenicol
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Lycett G W
Orr E
Pritchard R H
References (32)
32 references, click to expand
  1. Origin and sequence of chromosome replication in Escherichia coli.
    J Mol Biol. 1972 Oct 14;70(3):549-66 PMID: 4563262
  2. Control of the initiation of DNA replication in Escherichia coli. I. Negative control of initiation.
    Mol Gen Genet. 1979 Jan 10;168(2):185-95 PMID: 377010
  3. More precise mapping of the replication origin in Escherichia coli K-12.
    J Bacteriol. 1974 Oct;120(1):1-5 PMID: 4607435
  4. Chloramphenicol-induced changes in the synthesis of ribosomal, transfer, and messenger ribonucleic acids in Escherichia coli B/r.
    J Bacteriol. 1977 Jun;130(3):1098-108 PMID: 324974
  5. NOVEL Escherichia coli dnaB mutant: direct involvement of the dnaB252 gene product in the synthesis of an origin-ribonucleic acid species during initiaion of a round of deoxyribonucleic acid replication.
    J Bacteriol. 1977 Mar;129(3):1476-86 PMID: 321430
  6. The generation of a ColE1-Apr cloning vehicle which allows detection of inserted DNA.
    Mol Gen Genet. 1975 Dec 30;142(3):239-49 PMID: 1045010
  7. A simple method for the preparation of large quantities of pure plasmid DNA.
    Biochim Biophys Acta. 1975 Apr 2;383(4):457-63 PMID: 1092355
  8. Nature of Col E 1 plasmid replication in Escherichia coli in the presence of the chloramphenicol.
    J Bacteriol. 1972 May;110(2):667-76 PMID: 4336693
  9. The effects of an Escherichia coli dnaAts mutation on the replication of the plasmids colE1 pSC101, R100.1 and RTF-TC.
    Mol Gen Genet. 1979 Jul 13;174(2):117-26 PMID: 386040
  10. Suppression of the DnaA phenotype by mutations in the rpoB cistron of ribonucleic acid polymerase in Salmonella typhimurium and Escherichia coli.
    J Bacteriol. 1977 May;130(2):577-82 PMID: 400782
  11. A colony bank containing synthetic Col El hybrid plasmids representative of the entire E. coli genome.
    Cell. 1976 Sep;9(1):91-9 PMID: 788919
  12. Diameter of cells of a thermosensitive dnaA mutant of Escherichia coli cultivated at intermediate temperatures.
    J Bacteriol. 1978 Aug;135(2):575-80 PMID: 355234
  13. Biochemical construction of specific chimeric plasmids from ColE1 DNA and unfractionated Escherichia coli DNA.
    Proc Natl Acad Sci U S A. 1976 Nov;73(11):3838-42 PMID: 792875
  14. A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid.
    Biochem J. 1956 Feb;62(2):315-23 PMID: 13293190
  15. Purification and characterization of covalently closed replicative intermediates of ColEl DNA from Escherichia coli.
    Biochemistry. 1977 Apr 19;16(8):1677-83 PMID: 322703
  16. [Mutants of E. coli K 12 unable to grow on methyl-beta-D-glucuronide: map l ocation of uid A. locus of the structural gene of beta-D-glucuronidase].
    Mol Gen Genet. 1973;120(4):319-35 PMID: 4568840
  17. The control of ribonucleic acid synthesis in bacteria. The synthesis and stability of ribonucleic acid in chloramphenicol-inhibited cultures of Escherichia coli.
    Biochem J. 1971 Apr;122(2):149-59 PMID: 4940606
  18. In situ hybridization of "nick-translated" 3H-ribosomal DNA to chromosomes from salamanders.
    Chromosoma. 1976 Jan 27;54(1):15-25 PMID: 1248332
  19. Evidence for the direct involvement of RNA in the initiation of DNA replication in Escherichia coli 15T.
    J Mol Biol. 1972 Feb 28;64(1):47-60 PMID: 4552485
  20. Lambdoid phages that simplify the recovery of in vitro recombinants.
    Mol Gen Genet. 1977 Jan 7;150(1):53-61 PMID: 319344
  21. Size variations and correlation of different cell cycle events in slow-growing Escherichia coli.
    J Bacteriol. 1978 May;134(2):423-33 PMID: 350822
  22. Temporal sequence of events during the initiation process in Escherichia coli deoxyribonucleic acid replication: roles of the dnaA and dnaC gene products and ribonucleic acid polymerase.
    J Bacteriol. 1977 Mar;129(3):1466-75 PMID: 321429
  23. Stimulation by cyclic adenosine monophosphate of plasmid deoxyribonucleic acid replication and catabolite repression of the plasmid deoxyribonucleic acid-protein relaxation complex.
    J Bacteriol. 1973 May;114(2):577-91 PMID: 4350343
  24. Factor necessary for ribosomal RNA synthesis.
    Nature. 1970 Nov 21;228(5273):748-51 PMID: 4919595
  25. Pedigrees of some mutant strains of Escherichia coli K-12.
    Bacteriol Rev. 1972 Dec;36(4):525-57 PMID: 4568763
  26. Cloning of replication origins from the E. coli K12 chromosome.
    Nature. 1978 Oct 12;275(5680):561-4 PMID: 357995
  27. Regulation of the dnaA product in Escherichia coli.
    Mol Gen Genet. 1977 Oct 20;155(2):219-25 PMID: 337131
  28. Suppression of initiation-negative strains of Escherichia coli by integration of the sex factor F.
    J Bacteriol. 1975 Feb;121(2):554-61 PMID: 1089635
  29. Thermosensitive mutants of E. coli affected in the processes of DNA synthesis and cellular division.
    Cold Spring Harb Symp Quant Biol. 1968;33:677-93 PMID: 4892005
  30. A suitable method for construction and cloning hybrid plasmids containing EcoRI-fragments of E. coli genome.
    Mol Gen Genet. 1977 Jan 18;150(2):211-9 PMID: 320457
  31. Replication of plasmid pSC101 in Escherichia coli K12: requirement for dnaA function.
    Mol Gen Genet. 1977 Sep 9;154(3):225-30 PMID: 337104
  32. Effect of dna mutations on the replication of plasmid pSC101 in Escherichia coli K-12.
    J Bacteriol. 1979 Mar;137(3):1095-9 PMID: 374337
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00270480
Published
1980-00-00
Pages
329-36
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00270480
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2