Glomerular basement membrane synthesis and serum concentration of type IV collagen in streptozotocin-diabetic rats.

Diabetologia ·Vol. 26 ·No. 2 ·1984-02-00 ·Pages 150-4

Hasslacher C, Reichenbacher R, Gechter F, Timpl R

Abstract

Glomerular basement membrane synthesis was measured in vivo in diabetic rats at various times after streptozotocin injection. Simultaneously the type IV collagen concentration in serum was determined by a radioimmunoassay specific for the terminal cross-linking domain, 7S collagen. Basement membrane collagen synthesis and the 7S collagen levels were markedly raised in diabetic animals 18-34 days after streptozotocin injection and showed a significant correlation. The results suggest that the amount of serum 7S collagen reflects increased basement membrane synthesis in a diabetic situation. Both basement membrane collagen synthesis and serum 7S collagen concentration also show a positive correlation with the blood glucose levels of diabetic rats. This indicates that the degree of metabolic dysregulation influences basement membrane synthesis.

MeSH Terms

Age Factors Animals Basement Membrane/metabolism Blood Glucose/metabolism Collagen/biosynthesis,blood,metabolism Diabetes Mellitus, Experimental/blood,metabolism Hydroxyproline/metabolism Kidney Glomerulus/metabolism Male Proline/metabolism Radioimmunoassay Rats Rats, Inbred Strains Time Factors

Chemicals

Blood Glucose Collagen Proline Hydroxyproline

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Hasslacher C

Reichenbacher R

Gechter F

Timpl R

References (20)

20 references, click to expand

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Article Info

Journal

Diabetologia

Abbr.

Diabetologia

ISSN

0012-186X

DOI

10.1007/BF00281124

Published

1984-02-00

Pages

150-4

Language

English

Region

Germany

NLM ID

0006777

Subset

External Links

PubMed source DOI: 10.1007/BF00281124 View journal details

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Glomerular basement membrane synthesis and serum concentration of type IV collagen in streptozotocin-diabetic rats.

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