Soluble endoribonuclease activity from vaccinia virus: specific cleavage of virion-associated high-molecular-weight RNA.
A soluble endoribonuclease activity was extracted from purified vaccinia virus cores by treatment with sodium-deoxycholate and dithiothreitol. The soluble enzyme readily cleaved purified virion-associated high-molecular-weight RNA to limit-sized fragments sedimenting at 8 to 12S. Purified virion-released 8 to 12S polyadenylated mRNA was not degraded by the enzyme extract. The soluble endoribonuclease did not require the presence of ribonucleoside triphosphates for activity.
7 references, click to expand
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High molecular weight virion-associated RNA of vaccinia. A possible precursor to 8 to 12 S mRNA.J Biol Chem. 1977 Feb 10;252(3):872-7 PMID: 838702
-
Transcriptional complexity of vaccinia virus in vivo and in vitro.J Virol. 1977 Sep;23(3):608-15 PMID: 894791
-
Methyl group analysis of virion-associated high-molecular-weight RNA synthesized in vitro by purified vaccinia virus.J Virol. 1977 Jul;23(1):110-6 PMID: 886646
-
In vitro synthesis of a high molecular weight virion-associated RNA by vaccinia.J Biol Chem. 1977 Feb 10;252(3):866-71 PMID: 838701
-
Two nucleid acid-dependent nucleoside triphosphate phosphohydrolases from vaccinia virus. Purification and characterization.J Biol Chem. 1974 May 25;249(10):3273-80 PMID: 4364421
-
An intermediate in the synthesis of poliovirus RNA.Proc Natl Acad Sci U S A. 1966 Aug;56(2):741-8 PMID: 4291014
-
Ribonucleic acid synthesis in vaccinia virus. I. The mechanism of synthesis and release of RNA in vaccinia cores.J Mol Biol. 1970 May 28;50(1):1-18 PMID: 5453356
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