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PMID: 886646 Published · ppublish English Journal Article

Methyl group analysis of virion-associated high-molecular-weight RNA synthesized in vitro by purified vaccinia virus.

Journal of virology ·Vol. 23 ·No. 1 ·1977-07-00 ·Pages 110-6

Nuss DL, Paoletti E

Abstract

The methylation pattern of virion-associated high-molecular-weight RNA synthesized in vitro by purified vaccinia virus has been determined. Analysis of purified high-molecular-weight RNA synthesized with S-[methyl-3H]-adenosylmethionine and alpha[32P]UTP as precursors gave the following results. (i) Eessentially all molecules contained blocked and methylated structures of the type m7G(5')ppp(5')Gm and m7G(5')ppp(5')Am. (ii) There was no detectable methylation at internal sites. (iii) Under several different conditions of synthesis, the ratio of molecules containing m7G(5')ppp(5')Gm to those containing m7G(5')ppp(5')Am was imilar for both the virion-associated high-molecular-weight RNA and the virion-released 8-12S mRNA.

MeSH Terms
Base Sequence Cell-Free System HeLa Cells Methylation Molecular Weight RNA, Messenger/biosynthesis RNA, Viral/biosynthesis S-Adenosylmethionine/metabolism Uracil Nucleotides/metabolism Vaccinia virus/metabolism
Chemicals
RNA, Messenger RNA, Viral Uracil Nucleotides S-Adenosylmethionine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Nuss D L
Paoletti E
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22 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.23.1.110-116.1977
Published
1977-07-00
Pages
110-6
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC515806
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.23.1.110-116.1977 PMC Full Text View journal details
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