Molecular relationships of degradative plasmids determined by in situ hybridisation of their endonuclease-generated fragments.

Molecular & general genetics : MGG ·Vol. 160 ·No. 3 ·1978-04-17 ·Pages 347-51

Heinaru AL, Duggleby CJ, Broda P

Abstract

Plasmid inter-relationships were studied by hybridisation of a radioactively labelled DNA probe to endonuclease-derived fragmentation patterns of plasmids bound to a nitrocellulose filter. The degradative plasmids SAL and NAH were found to be very closely related, but probably one did not give rise to the other by just a single deletion or insertion. Relationships between SAL and other degradative plasmids are complex; substantial homology was found with TOL and other plasmids encoding toluate dissimilation and significant homology was found with OCT.

MeSH Terms

DNA, Bacterial/metabolism DNA, Single-Stranded/metabolism Electrophoresis, Agar Gel Endonucleases/metabolism Methods Nucleic Acid Hybridization Plasmids Pseudomonas/ultrastructure

Chemicals

DNA, Bacterial DNA, Single-Stranded Endonucleases

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Heinaru A L

Duggleby C J

Broda P

References (15)

15 references, click to expand

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Article Info

Journal

Molecular & general genetics : MGG

Abbr.

Mol Gen Genet

ISSN

0026-8925

DOI

10.1007/BF00332979

Published

1978-04-17

Pages

347-51

Language

English

Region

Germany

NLM ID

0125036

Subset

External Links

PubMed source DOI: 10.1007/BF00332979

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Molecular relationships of degradative plasmids determined by in situ hybridisation of their endonuclease-generated fragments.

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