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PMID: 6738533 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Repeated consensus sequence and pseudopromoters in the four coordinately regulated tubulin genes of Chlamydomonas reinhardi.

Molecular and cellular biology ·Vol. 4 ·No. 6 ·1984-06-00 ·Pages 1115-24

Brunke KJ, Anthony JG, Sternberg EJ, Weeks DP

Abstract

The 5' coding and promoter regions of the four coordinately regulated tubulin genes of Chlamydomonas reinhardi have been mapped and sequenced. DNA sequencing data shows that the predicted N-terminal amino acid sequences of Chlamydomonas alpha- and beta-tubulins closely match that of tubulins of other eucaryotes. Within the alpha 1- and alpha 2-tubulin gene set and the beta 1- and beta 2-tubulin gene set, both nucleotide sequence and intron placement are highly conserved. Transcription initiation sites have been located by primer extension analysis at 140, 141, 159, and 132 base pairs upstream of the translation initiator codon for the alpha 1-, alpha 2-, beta 1-, and beta 2-tubulin genes, respectively. Among the structures with potential regulatory significance, the most striking is a 16-base-pair consensus sequence [GCTC(G/C)AAGGC(G/T)(G/C)--(C/A)(C/A)G] which is found in multiple copies immediately upstream of the TATA box in each of the four genes. An unexpected discovery is the presence of pseudopromoter regions in two of the transcribed tubulin genes. One pseudopromoter region is located 400 base pairs upstream of the authentic alpha 2-tubulin gene promoter, whereas the other is located within the transcribed 5' noncoding region of the beta 1-tubulin gene.

MeSH Terms
Amino Acid Sequence Base Sequence Chlamydomonas/genetics,metabolism Genes Genes, Regulator Operon Repetitive Sequences, Nucleic Acid Transcription, Genetic Tubulin/genetics
Chemicals
Tubulin
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Brunke K J
Anthony J G
Sternberg E J
Weeks D P
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.4.6.1115-1124.1984
Published
1984-06-00
Pages
1115-24
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC368880
Subset
IM
Databases
GENBANK
K01805, K01807, K01808, K01809
External Links
PubMed source DOI: 10.1128/mcb.4.6.1115-1124.1984 PMC Full Text View journal details
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