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PMID: 6739293 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Binding of ethidium and bis(methidium)spermine to Z DNA by intercalation.

Nucleic acids research ·Vol. 12 ·No. 11 ·1984-06-11 ·Pages 4679-90

Shafer RH, Brown SC, Delbarre A, Wade D

Abstract

The interaction of ethidium bromide, a DNA intercalating drug, and bis( methidium )spermine, a DNA bis-intercalating compound, with the left-handed Z form of poly(dG-dC) has been studied in 4.4 M NaCl. Spectrophotometric analysis using absorption, fluorescence and circular dichroism indicates that the complex formed between ethidium and Z DNA resembles very closely that formed with B DNA. This suggests that ethidium binds to Z DNA by intercalation. 31P NMR spectra are presented showing both the conversion of the Z form to the B form with increasing amounts of drug and the typical Z form spectrum at low binding densities. Data are also presented which show that the bifunctional intercalator bis( methidium )spermine binds to Z DNA in a manner similar to its binding to B DNA, i.e., by bis-intercalation. These results are important for our understanding the behavior of Z DNA and its biological significance.

MeSH Terms
Animals Cattle Circular Dichroism DNA Ethidium/analogs & derivatives Intercalating Agents Magnetic Resonance Spectroscopy Nucleic Acid Conformation Polydeoxyribonucleotides Spectrometry, Fluorescence Spectrophotometry, Ultraviolet Thymus Gland
Chemicals
Intercalating Agents Polydeoxyribonucleotides poly(dC-dG) bismethidium spermine DNA Ethidium
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Shafer R H
Brown S C
Delbarre A
Wade D
References (32)
32 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/12.11.4679
Published
1984-06-11
Pages
4679-90
Language
English
Region
England
NLM ID
0411011
PMCID
PMC318867
Subset
IM
Grants
NCI NIH HHS · CA 27343 · United States
External Links
PubMed source DOI: 10.1093/nar/12.11.4679 PMC Full Text View journal details
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