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PMID: 6739294 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

DNA sequence and transcription of a DNA minicircle isolated from male-fertile sugar beet mitochondria.

Nucleic acids research ·Vol. 12 ·No. 11 ·1984-06-11 ·Pages 4747-56

Hansen BM, Marcker KA

Abstract

A minicircle consisting of 1440 bp was isolated from male fertile sugar beet mitochondria. The entire nucleotide sequence of the minicircle was determined. There are several stop codons in all three reading frames and it is therefore unlikely that the minicircle codes for a polypeptide product. However, the minicircle is transcribed yielding a major RNA product consisting of 850 bases in addition to two very minor RNA products of 1100 and 950 bases, respectively. All three RNA products are transcribed from the same strand. The starting point for transcription of the major RNA product was determined by S1 nuclease protection analysis.

MeSH Terms

Base Composition Cloning, Molecular DNA, Circular/genetics DNA, Mitochondrial/genetics Fertilization Male Mitochondria/metabolism Nucleic Acid Hybridization Plants/genetics Plasmids Transcription, Genetic

Chemicals

DNA, Circular DNA, Mitochondrial

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Hansen B M

Marcker K A

References (13)

13 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/12.11.4747

Published

1984-06-11

Pages

4747-56

Language

English

Region

England

NLM ID

0411011

PMCID

PMC318872

Subset

Databases

GENBANK

X00641

External Links

PubMed source DOI: 10.1093/nar/12.11.4747 PMC Full Text View journal details

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DNA sequence and transcription of a DNA minicircle isolated from male-fertile sugar beet mitochondria.

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