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PMID: 6746574 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Subcellular localization of alkaline phosphatase in Bacillus licheniformis 749/C by immunoelectron microscopy with colloidal gold.

Journal of bacteriology ·Vol. 159 ·No. 2 ·1984-08-00 ·Pages 668-77

Tinglu G, Ghosh A, Ghosh BK

Abstract

Subcellular distribution of the alkaline phosphatase of Bacillus licheniformis 749/C was determined by an immunoelectron microscopy method. Anti-alkaline phosphatase antibody labeled with 15- to 18-nm colloidal gold particles (gold-immunoglobulin G [IgG] complex) were used for the study. Both the plasma membrane and cytoplasmic material were labeled with the gold-IgG particles. These particles formed clusters in association with the plasma membrane; in contrast, in the cytoplasm the particles were largely dispersed, and only a few clusters were found. The gold-IgG binding was quantitatively estimated by stereological analysis of labeled, frozen thin sections. This estimation of a variety of control samples showed that the labeling was specific for the alkaline phosphatase. Cluster formation of the gold-IgG particles in association with the plasma membrane suggests that existence of specific alkaline phosphatase binding sites (receptors) in the plasma membrane of B. licheniformis 749/C.

MeSH Terms
Alkaline Phosphatase/metabolism Bacillus/enzymology,ultrastructure Cell Membrane/enzymology Gold Histocytochemistry Immunoassay Immunoglobulin G Indicators and Reagents Kinetics Microscopy, Electron Subcellular Fractions/enzymology,ultrastructure
Chemicals
Immunoglobulin G Indicators and Reagents Gold Alkaline Phosphatase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Tinglu G
Ghosh A
Ghosh B K
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22 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.159.2.668-677.1984
Published
1984-08-00
Pages
668-77
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC215696
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.159.2.668-677.1984 PMC Full Text View journal details
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