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PMID: 6749811 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Cloning of trg, a gene for a sensory transducer in Escherichia coli.

Journal of bacteriology ·Vol. 152 ·No. 1 ·1982-10-00 ·Pages 372-83

Harayama S, Engström P, Wolf-Watz H, Iino T, Hazelbauer GL

Abstract

Clones of trg, a gene which codes for a chemotactic transducer, were isolated linked to ColE1 and pBR322 vectors. Studies with the hybrid plasmids demonstrated unequivocally that trg is the structural gene for methyl-accepting chemotaxis protein III. The Trg protein was found to be structurally complex, electrophoresing as a series of seven bands on high-resolution sodium dodecyl sulfate-polyacrylamide gels. The multiplicity of bands is a function of the activity of cheR, which codes for a methyltransferase, and of cheB, which codes for a demethylase. It appears that Trg, a quantitatively minor transducer, resembles the two major transducer proteins, Tsr and Tar, in that all three are multiply methylated and also multiply modified in a second way which requires an active cheB gene. However, preliminary analysis of the Trg protein indicated that it is significantly less related structurally to the Tsr or Tar protein than those two transducers are to each other. This implies that the features of multiple methylation and cheB-dependent modification are likely to be critical for the common physiological functions in chemotactic excitation and adaptation performed by all three transducers.

MeSH Terms
Bacterial Proteins/genetics Cloning, Molecular Escherichia coli/genetics,metabolism Genes Membrane Proteins Methyl-Accepting Chemotaxis Proteins Methyltransferases/analysis,biosynthesis,genetics Plasmids
Chemicals
Bacterial Proteins Membrane Proteins Methyl-Accepting Chemotaxis Proteins tsr protein, E coli Methyltransferases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Harayama S
Engström P
Wolf-Watz H
Iino T
Hazelbauer G L
References (49)
49 references, click to expand
  1. On the production of deletions in the chromosome of Escherichia coli.
    J Mol Biol. 1970 Oct 14;53(1):49-67 PMID: 4922221
  2. Plasmid ColEl as a molecular vehicle for cloning and amplification of DNA.
    Proc Natl Acad Sci U S A. 1974 Sep;71(9):3455-9 PMID: 4610576
  3. Production, properties and utility of bacterial minicells.
    Curr Top Microbiol Immunol. 1975;69:1-84 PMID: 1098854
  4. Transient response to chemotactic stimuli in Escherichia coli.
    Proc Natl Acad Sci U S A. 1975 Aug;72(8):3235-9 PMID: 1103143
  5. A colony bank containing synthetic Col El hybrid plasmids representative of the entire E. coli genome.
    Cell. 1976 Sep;9(1):91-9 PMID: 788919
  6. Cleavage map of colicin E1 plasmid.
    Nature. 1976 Nov 11;264(5582):193-6 PMID: 792714
  7. Identification of a protein methyltransferase as the cheR gene product in the bacterial sensing system.
    Proc Natl Acad Sci U S A. 1977 Feb;74(2):533-7 PMID: 322131
  8. Sensory transduction in Escherichia coli: two complementary pathways of information processing that involve methylated proteins.
    Proc Natl Acad Sci U S A. 1977 Aug;74(8):3312-6 PMID: 333433
  9. Chemotaxis in Escherichia coli: methylation of che gene products.
    Proc Natl Acad Sci U S A. 1977 Aug;74(8):3317-21 PMID: 333434
  10. Bacterial flagella.
    Annu Rev Microbiol. 1977;31:397-419 PMID: 410356
  11. Synthesis of mot and che gene products of Escherichia coli programmed by hybrid ColE1 plasmids in minicells.
    J Bacteriol. 1977 Dec;132(3):996-1002 PMID: 336614
  12. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
    Gene. 1977;2(2):95-113 PMID: 344137
  13. A protein methylesterase involved in bacterial sensing.
    Proc Natl Acad Sci U S A. 1978 Aug;75(8):3659-63 PMID: 358191
  14. Transposon-insertion mutants of Escherichia coli K12 defective in a component common to galactose and ribose chemotaxis.
    Mol Gen Genet. 1979 Mar 20;171(2):193-203 PMID: 375029
  15. Mutants in transmission of chemotactic signals from two independent receptors of E. coli.
    Cell. 1979 Mar;16(3):617-25 PMID: 378395
  16. Relation of chemotactic response to the amount of receptor: evidence for different efficiencies of signal transduction.
    J Bacteriol. 1979 Jun;138(3):739-47 PMID: 378935
  17. Protein methylation in behavioural control mechanisms and in signal transduction.
    Nature. 1979 Jul 26;280(5720):279-84 PMID: 379649
  18. Studies on bacterial chemotaxis. IV. Interaction of maltose receptor with a membrane-bound chemosensing component.
    J Biochem. 1979 Jul;86(1):27-34 PMID: 383708
  19. Isolation and characterization of DNA repetitions carrying the chromosomal beta-lactamase gene of Escherichia coli K-12.
    Mol Gen Genet. 1979 Jun 7;173(2):115-25 PMID: 386030
  20. Parallel pathways for transduction of chemotactic signals in Escherichia coli.
    Nature. 1980 Jan 3;283(5742):98-100 PMID: 6985714
  21. A restriction enzyme cleavage map of Tn5 and location of a region encoding neomycin resistance.
    Mol Gen Genet. 1979;177(1):65-72 PMID: 231729
  22. New map of bacteriophage lambda DNA.
    J Virol. 1980 Jan;33(1):390-400 PMID: 6245240
  23. Cloning and restriction mapping of the trmA gene coding for transfer ribonucleic acid (5-methyluridine)-methyltransferase in Escherichia coli K-12.
    J Bacteriol. 1980 May;142(2):371-9 PMID: 6247318
  24. Tumbling chemotaxis mutants of Escherichia coli: possible gene-dependent effect of methionine starvation.
    J Bacteriol. 1980 May;142(2):527-34 PMID: 6991478
  25. Construction of a physical map of a kanamycin (Km) transposon, Tn5, and a comparison to another Km transposon, Tn903.
    Mol Gen Genet. 1980 Apr;178(1):77-83 PMID: 6247620
  26. Multiple methylation of methyl-accepting chemotaxis proteins during adaptation of E. coli to chemical stimuli.
    Cell. 1980 May;20(1):165-71 PMID: 6993007
  27. Definition of additional flagellar genes in Escherichia coli K12.
    Genetics. 1980 Feb;94(2):277-90 PMID: 6993282
  28. Studies on bacterial chemotaxis. V. Possible involvement of four species of the methyl-accepting chemotaxis protein in chemotaxis of Escherichia coli.
    J Biochem. 1980 May;87(5):1365-70 PMID: 6993458
  29. Multiple methylation in processing of sensory signals during bacterial chemotaxis.
    Proc Natl Acad Sci U S A. 1980 May;77(5):2429-33 PMID: 6446711
  30. Structural studies of methyl-accepting chemotaxis proteins of Escherichia coli: evidence for multiple methylation sites.
    Proc Natl Acad Sci U S A. 1980 May;77(5):2434-8 PMID: 6994098
  31. Genetic and biochemical properties of Escherichia coli mutants with defects in serine chemotaxis.
    J Bacteriol. 1980 Dec;144(3):1048-60 PMID: 6777365
  32. Multiple forms of methyl-accepting chemotaxis proteins distinguished by a factor in addition to multiple methylation.
    J Bacteriol. 1981 Jan;145(1):35-42 PMID: 7007319
  33. Methyl-accepting chemotaxis protein III and transducer gene trg.
    J Bacteriol. 1981 Jan;145(1):43-9 PMID: 7007323
  34. Multiple electrophoretic forms of methyl-accepting chemotaxis proteins generated by stimulus-elicited methylation in Escherichia coli.
    J Bacteriol. 1980 Aug;143(2):809-15 PMID: 6782079
  35. Receptor structure in the bacterial sensing system.
    Proc Natl Acad Sci U S A. 1980 Dec;77(12):7157-61 PMID: 6784119
  36. Energy is required for maturation of exported proteins in Escherichia coli.
    Eur J Biochem. 1981 May 15;116(2):227-33 PMID: 7018904
  37. The methyl-accepting chemotaxis proteins of E. coli: a repellent-stimulated, covalent modification, distinct from methylation.
    Cell. 1981 Aug;25(2):333-40 PMID: 7026041
  38. Posttranslational processing of methyl-accepting chemotaxis proteins in Escherichia coli.
    Proc Natl Acad Sci U S A. 1981 Oct;78(10):6051-5 PMID: 6458812
  39. Sensory transducers of E. coli are encoded by homologous genes.
    Cell. 1981 Nov;26(3 Pt 1):333-43 PMID: 7034961
  40. P1 transduction mapping of the trg locus in rac+ and rac strains of Escherichia coli K-12.
    J Bacteriol. 1982 Feb;149(2):529-33 PMID: 6276359
  41. A gene coding for a periplasmic protein is located near the locus for termination of chromosome replication in Escherichia coli.
    J Bacteriol. 1982 Sep;151(3):1391-6 PMID: 7050089
  42. Role of the galactose binding protein in chemotaxis of Escherichia coli toward galactose.
    Nat New Biol. 1971 Mar 24;230(12):101-4 PMID: 4927373
  43. The gradient-sensing mechanism in bacterial chemotaxis.
    Proc Natl Acad Sci U S A. 1972 Sep;69(9):2509-12 PMID: 4560688
  44. Chemotaxis in Escherichia coli analysed by three-dimensional tracking.
    Nature. 1972 Oct 27;239(5374):500-4 PMID: 4563019
  45. Flagellar assembly mutants in Escherichia coli.
    J Bacteriol. 1972 Nov;112(2):986-93 PMID: 4117584
  46. Escherichia coli K-12 F-prime factors, old and new.
    Bacteriol Rev. 1972 Dec;36(4):587-607 PMID: 4568765
  47. Change in direction of flagellar rotation is the basis of the chemotactic response in Escherichia coli.
    Nature. 1974 May 3;249(452):74-7 PMID: 4598031
  48. Identification of the ribose binding protein as the receptor for ribose chemotaxis in Salmonella typhimurium.
    Biochemistry. 1974 Oct 22;13(22):4473-8 PMID: 4609457
  49. Maltose chemoreceptor of Escherichia coli.
    J Bacteriol. 1975 Apr;122(1):206-14 PMID: 1091624
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.152.1.372-383.1982
Published
1982-10-00
Pages
372-83
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC221422
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.152.1.372-383.1982 PMC Full Text View journal details
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