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PMID: 6752115 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Characterization of eight beta-lactamases of Gram-negative bacteria.

Journal of bacteriology ·Vol. 152 ·No. 2 ·1982-11-00 ·Pages 567-71

Sawai T, Kanno M, Tsukamoto K

Abstract

Eight kinds of beta-lactamases produced by gram-negative bacteria were characterized by the following properties: molecular weight, isoelectric point, pH optimum, molecular activity, immunochemical reactivity, and kinetic parameters with respect to twelve kinds of common beta-lactam antibiotics. These beta-lactamases included two types of penicillinases mediated by R plasmids and six kinds of species-specific cephalosporinases. To determine a reliable value of the kinetic parameter, Km, we introduced a continuous and acidimetric assay method of beta-lactamase activity with a pH stat.

MeSH Terms
Citrobacter/enzymology Enterobacter/enzymology Enterobacteriaceae/enzymology Escherichia coli/enzymology Kinetics Proteus/enzymology Serratia marcescens/enzymology Species Specificity Substrate Specificity beta-Lactamases/metabolism
Chemicals
beta-Lactamases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Sawai T
Kanno M
Tsukamoto K
References (22)
22 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.152.2.567-571.1982
Published
1982-11-00
Pages
567-71
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC221503
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.152.2.567-571.1982 PMC Full Text View journal details
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