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PMID: 6754742 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Topography of reaction center subunits in the membrane of the photosynthetic bacterium, rhodopseudomonas sphaeroides.

The Journal of cell biology ·Vol. 95 ·No. 1 ·1982-10-00 ·Pages 179-88

Valkirs GE, Feher G

Abstract

The localization of the reaction center polypeptides (L, M, and H) in the membranes of both the wild-type, strain 2.4.1, and the carotenoidless mutant, R-26, of Rhodopseudomonas sphaeroides was determined by using affinity-purified antibodies specific for these proteins. Binding of the antibodies to reaction center subunits in spheroplasts was visualized in the electron microscope by immunoferritin labeling. The H and M subunits were labeled at both the cytoplasmic and the periplasmic surfaces of the membrane, whereas the L subunit was labeled only at the periplasmic surface of the membrane. Thus, the reaction center is asymmetrically oriented in the membrane with at least two subunits (H and M) spanning the membrane.

MeSH Terms
Antibodies, Bacterial Antibody Specificity Bacterial Proteins/immunology Cell Membrane/ultrastructure Immunologic Techniques Macromolecular Substances Membrane Proteins/immunology Microscopy, Electron Rhodobacter sphaeroides/ultrastructure
Chemicals
Antibodies, Bacterial Bacterial Proteins Macromolecular Substances Membrane Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Valkirs G E
Feher G
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.95.1.179
Published
1982-10-00
Pages
179-88
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2112351
Subset
IM
Grants
NIGMS NIH HHS · GM 13191 · United States
External Links
PubMed source DOI: 10.1083/jcb.95.1.179 PMC Full Text View journal details
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