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PMID: 6755394 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Regulation of transcription from tandem and convergent promoters.

Nucleic acids research ·Vol. 10 ·No. 18 ·1982-09-25 ·Pages 5447-65

Horowitz H, Platt T

Abstract

We have examined transcription on templates containing the trp and lac UV5 promoters arranged in tandem or opposing orientations. These studies have revealed that the strengths of the two promoters are comparable, though the lac UV5 promoter is much more sensitive to the level of initiating purine present. Kinetic experiments have shown that a polymerase molecule poised at the lac promoter, or a lac repressor molecule bound to the lac operator, can temporarily block a polymerase molecule initiated from the trp promoter, though transcription eventually continues through. In the convergent construct, transcription from the lac promoter is hindered only when initiation is suboptimal due to low purine concentrations.

MeSH Terms
Base Sequence DNA-Directed RNA Polymerases/antagonists & inhibitors Escherichia coli/genetics Kinetics Lac Operon Operon Plasmids Purine Nucleotides/metabolism Repressor Proteins/metabolism Templates, Genetic Transcription, Genetic
Chemicals
Purine Nucleotides Repressor Proteins DNA-Directed RNA Polymerases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Horowitz H
Platt T
References (23)
23 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/10.18.5447
Published
1982-09-25
Pages
5447-65
Language
English
Region
England
NLM ID
0411011
PMCID
PMC320888
Subset
IM
Grants
NIGMS NIH HHS · GM-22830 · United States
External Links
PubMed source DOI: 10.1093/nar/10.18.5447 PMC Full Text View journal details
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