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PMID: 6761542 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Isolation and characterization of an operator-constitutive mutation in the recA gene of E. coli K-12.

Molecular & general genetics : MGG ·Vol. 187 ·No. 1 ·1982-00-00 ·Pages 4-11

Ginsburg H, Edmiston SH, Harper J, Mount DW

Abstract

The recA gene of E. coli is regulated by a specific repressor, the lexA protein, which binds to an operator in the recA regulatory region. We describe in this paper the isolation and characterization of a mutant thought to carry an operator-constitutive mutation in the recA gene. This mutation has the following properties: 1) It partially suppresses the UV sensitivity of lexA- strains. 2) It maps near the recA gene. 3) It allows constitutive high-level synthesis of recA protein in both lexA- and lexA+ backgrounds. 4) It allows constitutive synthesis of the recA messenger RNA. 5) It is cis-acting. The mutation does not restore induced cellular mutagenesis in a lexA- background. The expression of induced repair and mutagenesis of UV irradiated phage lambda or the regulation of the lexA gene is not affected by the presence of the mutation in either a lexA+ or lexA- strain. These observations confirm other findings that high levels of recA protein synthesis per se is not sufficient for the expression of UV inducible functions and that the lexA protein represses other genes besides the recA gene.

MeSH Terms
Autoradiography Electrophoresis, Polyacrylamide Gel Escherichia coli/genetics,radiation effects Genes, Bacterial/radiation effects Mutation Operon/radiation effects Plasmids/radiation effects Ultraviolet Rays
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Ginsburg H
Edmiston S H
Harper J
Mount D W
References (44)
44 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00384376
Published
1982-00-00
Pages
4-11
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Grants
NIGMS NIH HHS · GM24496 · United States
External Links
PubMed source DOI: 10.1007/BF00384376
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