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PMID: 6765234 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Is there proofreading during polypeptide synthesis?

The EMBO journal ·Vol. 1 ·No. 6 ·1982-00-00 ·Pages 741-5

Ruusala T, Ehrenberg M, Kurland CG

Abstract

The stoichiometric efficiency with which ternary complexes containing Phe-tRNAphe and Leu-tRNAleu support polypeptide synthesis has been compared in a poly(U)-directed, steady-state translation system. When unfractionated tRNA is used to support synthesis, the number of discharged ternary complexes per peptide bond formed is an average of 48 times greater for leucine than for phenylalanine. When three purified leucine isoacceptor species are tested, they each show a characteristic ratio of ternary complexes discharged per missense insertion, normalized to that for phenylalanine: these are 103, 76, and 45 for Leu- tRNA2leu , Leu- tRNA3leu , and Leu- tRNA4leu , respectively. The data are consistent with the functioning of a proofreading mechanism during translation.

MeSH Terms
Escherichia coli/genetics Kinetics Leucine/metabolism Models, Genetic Peptide Chain Elongation, Translational Peptide Elongation Factor Tu Peptide Elongation Factors/metabolism Peptides/genetics Phenylalanine/metabolism Poly U/metabolism Protein Biosynthesis RNA, Transfer, Amino Acyl/metabolism Ribosomes/metabolism
Chemicals
Peptide Elongation Factors Peptides RNA, Transfer, Amino Acyl Poly U Phenylalanine Peptide Elongation Factor Tu Leucine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Ruusala T
Ehrenberg M
Kurland C G
References (23)
23 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1982-00-00
Pages
741-5
Language
English
Region
England
NLM ID
8208664
PMCID
PMC553278
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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