Abstract
The influence of a cloned trpE (trpE+p) gene from Bacillus pumilus on the expression of the gat locus in Bacillus subtilis was examined. The trpE gene was regulated by tryptophan and the mtr locus, which specifies the presumed aporepressor. The specific activity of subunit G varied directly with the level of subunit Ep, and the heterologous EpG complex that was formed was stable to gel filtration.
MeSH Terms
Anthranilate Synthase
Bacillus/genetics
Bacillus subtilis/genetics
Cell-Free System
Genes, Regulator
Glutamine/genetics,metabolism
Mutation
Nitrogenous Group Transferases
Transferases/genetics,metabolism
Transformation, Bacterial
Tryptophan/genetics
Chemicals
Glutamine
Tryptophan
Transferases
Nitrogenous Group Transferases
Anthranilate Synthase
anthranilate synthase, glutamine amidotransferase subunit
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kane J F
Hill R J
References (11)
11 references, click to expand
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