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PMID: 6768357 Published · ppublish English Journal Article

Metabolism of hydrogen peroxide in Euglena gracilis Z by L-ascorbic acid peroxidase.

The Biochemical journal ·Vol. 186 ·No. 1 ·1980-01-15 ·Pages 377-80

Shigeoka S, Nakano Y, Kitaoka S

Abstract

Euglena gracilis was found to contain a peroxidase that specifically require L-ascorbic acid as the natural electron donor in the cytosol. The presence of an oxidation-reduction system metabolizing L-ascorbic acid was demonstrated in Euglena cells. Oxidation of L-ascorbic acid by the peroxidase, and the absence of ascorbic acid oxidase activity, suggests that the system functions to remove H2O2 in E. gracilis, which lacks catalase.

MeSH Terms
Ascorbate Peroxidases Euglena gracilis/enzymology Hydrogen Peroxide/metabolism Oxidation-Reduction Peroxidases/metabolism Pyrogallol/metabolism Subcellular Fractions/enzymology
Chemicals
Pyrogallol Hydrogen Peroxide Peroxidases Ascorbate Peroxidases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Shigeoka S
Nakano Y
Kitaoka S
References (24)
24 references, click to expand
  1. A spectroscopic and kinetic investigation of anion binding to ascorbate oxidase.
    Biochemistry. 1977 May 3;16(9):1926-9 PMID: 857881
  2. Ascorbic acid and photosynthesis. I. Monodehydroascorbic acid reductase of chloroplasts.
    Biochim Biophys Acta. 1958 Dec;30(3):453-7 PMID: 13618249
  3. A new method for simultaneous purification of cytochrome b5 and NADPH-cytochrome c reductase from rat liver microsomes.
    J Biochem. 1970 Feb;67(2):249-57 PMID: 4392647
  4. Identification, by electron paramagnetic resonance spectroscopy, of free radicals generated from substrates by peroxidase.
    J Biol Chem. 1960 Aug;235:2444-9 PMID: 13846434
  5. DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS.
    Ann N Y Acad Sci. 1964 Dec 28;121:404-27 PMID: 14240539
  6. Ascorbate oxidase and related copper proteins.
    Ann N Y Acad Sci. 1975 Sep 30;258:209-20 PMID: 812408
  7. Superoxide dismutases in photosynthetic organisms: absence of the cuprozinc enzyme in eukaryotic algae.
    Arch Biochem Biophys. 1977 Feb;179(1):243-56 PMID: 402888
  8. The reduction of cytochrome c by photosynthetic pyridine nucleotide reductase and transhydrogenase.
    Biochim Biophys Acta. 1962 Aug 13;62:417-20 PMID: 14463131
  9. The cellular production of hydrogen peroxide.
    Biochem J. 1972 Jul;128(3):617-30 PMID: 4404507
  10. Phytochrome-mediated synthesis of ascorbic acid oxidase in mustard cotyledons.
    Biochim Biophys Acta. 1974 Sep 5;362(2):258-65 PMID: 4422698
  11. Ferric and manganic superoxide dismutases in Euglena gracilis.
    Arch Biochem Biophys. 1979 Jul;195(2):535-45 PMID: 112927
  12. A role of mitochondrial glutathione peroxidase in modulating mitochondrial oxidations in liver.
    Eur J Biochem. 1978 Mar 15;84(2):377-83 PMID: 25178
  13. The intracellular localization of glycollate oxidoreductase in Euglena gracilis.
    Biochem J. 1971 Sep;124(2):275-81 PMID: 5003470
  14. Ascorbic acid and glutathione as respiratory carriers in the respiration of pea seedlings.
    Biochem J. 1956 Feb;62(2):248-59 PMID: 13293180
  15. Univalent reduction of molecular oxygen by spinach chloroplasts on illumination.
    J Biol Chem. 1974 Apr 10;249(7):2175-81 PMID: 4362064
  16. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  17. Separation and properties of the NAD-linked and NADP-linked isozymes of succinic semialdehyde dehydrogenase in Euglena gracilis z.
    Biochim Biophys Acta. 1976 Mar 11;429(1):55-62 PMID: 4121
  18. Subcellular localization of the GABA-shunt enzymes in Euglena gracilis strain Z.
    J Protozool. 1979 Aug;26(3):471-3 PMID: 119850
  19. The chloroplast at work. A review of modern developments in our understanding of chloroplast metabolism.
    Prog Biophys Mol Biol. 1978;33(1):1-54 PMID: 415334
  20. H2O2 destruction by ascorbate-dependent systems from chloroplasts.
    Biochim Biophys Acta. 1979 Jun 5;546(3):426-35 PMID: 454577
  21. The occurrence and fine structural characterization of microbodies in Euglena gracilis.
    Protoplasma. 1971;72(2):141-52 PMID: 5555984
  22. Peroxidative Activity in Euglena gracilis.
    Plant Physiol. 1975 Jun;55(6):1123-4 PMID: 16659224
  23. Occurrence and operation of the glycollate--glyoxylate shuttle in mitochondria of Euglena gracilis Z.
    Biochem J. 1979 Oct 15;184(1):189-92 PMID: 118746
  24. The biosynthetic pathway of L-ascorbic acid in Euglena gracilis Z.
    J Nutr Sci Vitaminol (Tokyo). 1979;25(4):299-307 PMID: 118242
Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1860377
Published
1980-01-15
Pages
377-80
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1161541
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1860377 PMC Full Text View journal details
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