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PMID: 6769902 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Plasmid-mediated transformation in Bacillus megaterium.

Journal of bacteriology ·Vol. 142 ·No. 2 ·1980-05-00 ·Pages 508-12

Brown BJ, Carlton BC

Abstract

A transformation system was developed for Bacillus megaterium by using antibiotic resistance plasmid deoxyribonucleic acid molecules derived from Staphylococcus aureus and Bacillus cereus. Lysozyme-generated protoplasts of B. megaterium allowed uptake of plasmid deoxyribonucleic acid in the presence of polyethylene glycol. Transformants expressed the antibiotic resistance determinants present on the plasmid deoxyribonucleic acid, and reisolated plasmid deoxyribonucleic acid yielded restriction endonuclease digestion patterns identical to those of the donor deoxyribonucleic acid.

MeSH Terms
Anti-Bacterial Agents/pharmacology Bacillus cereus/genetics Bacillus megaterium/genetics DNA, Bacterial/genetics Drug Resistance, Microbial Plasmids Polyethylene Glycols/pharmacology Protoplasts Staphylococcus aureus/genetics Transformation, Bacterial
Chemicals
Anti-Bacterial Agents DNA, Bacterial Polyethylene Glycols
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Brown B J
Carlton B C
References (10)
10 references, click to expand
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    J Bacteriol. 1973 May;114(2):625-31 PMID: 4196248
  2. Transformation in Bacillus subtilis. Fate of newly introduced transforming DNA.
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    Proc Natl Acad Sci U S A. 1976 Jun;73(6):2151-5 PMID: 819934
  4. Bacteriocin and antibiotic resistance plasmids in Bacillus cereus and Bacillus subtilis.
    J Bacteriol. 1978 Feb;133(2):897-903 PMID: 415051
  5. Characterization of Staphylococcus aureus plasmids introduced by transformation into Bacillus subtilis.
    J Bacteriol. 1978 Apr;134(1):318-29 PMID: 418061
  6. Construction and properties of chimeric plasmids in Bacillus subtilis.
    Proc Natl Acad Sci U S A. 1978 Mar;75(3):1428-32 PMID: 418412
  7. High frequency transformation of Bacillus subtilis protoplasts by plasmid DNA.
    Mol Gen Genet. 1979 Jan 5;168(1):111-5 PMID: 107388
  8. Polyethylene-glycol induced fusion of bacterial protoplasts: direct selection of recombinants.
    Mol Gen Genet. 1979 Jan 5;168(1):55-9 PMID: 107389
  9. Physical mapping of a plasmid from Bacillus megaterium by restriction endonuclease cleavage.
    Plasmid. 1979 Jan;2(1):59-68 PMID: 112602
  10. Interspecific induction of transformation of bacteria with respect to phage-susceptibility.
    Nature. 1961 Jun 3;190:937-8 PMID: 13702514
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.142.2.508-512.1980
Published
1980-05-00
Pages
508-12
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC294013
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.142.2.508-512.1980 PMC Full Text View journal details
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